Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress
Morchella protein hydrolysate (MPH) and its glycosylated derivative (G-MPH) may possess the potential as natural antioxidants. However, knowledge about the protective effects of MPH and G-MPH on cellular oxidative damage is limited. This study evaluated whether MPH and G-MPH protected Caco-2 cells f...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2019-08-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/pjfns/The-cytoprotective-effect-of-Morchella-esculenta-protein-hydrolysate-and-it-s-derivative,110134,0,2.html |
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| Summary: | Morchella protein hydrolysate (MPH) and its glycosylated derivative (G-MPH) may possess the potential as natural antioxidants. However, knowledge about the protective effects of MPH and G-MPH on cellular oxidative damage is limited. This study evaluated whether MPH and G-MPH protected Caco-2 cells from H2O2-induced oxidative injury and explored the potential mechanisms of protection. The results showed that, under H2O2 stress, both MPH and G-MPH significantly increased cell viability, suppressed intracellular ROS and MDA production, increased cellular antioxidant capacity, and activated Nrf2 signaling pathway. More importantly, MPH and G-MPH significantly inhibited the H2O2-induced apoptosis via restoring the loss of mitochondrial membrane potential and regulating the protein expressions of Bax, Bcl-2, and caspase-3. These data indicate that MPH and G-MPH can protect Caco-2 cells against oxidative injury by improving cellular antioxidant responses and inhibiting apoptosis. Therefore, MPH and G-MPH can have a broad application potential as promising ingredients of nutraceutical products or functional foods. |
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| ISSN: | 2083-6007 |