Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria
Aims. To synthesize, characterize, and analyze antimicrobial activity of AgNPs of Escherichia hermannii (SHE), Citrobacter sedlakii (S11P), and Pseudomonas putida (S5). Methods. The synthesized AgNPs were examined using ultraviolet-visible spectroscopy (UV-vis) and, zeta potential, and the size and...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/704708 |
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| author | Amr T. M. Saeb Ahmad S. Alshammari Hessa Al-Brahim Khalid A. Al-Rubeaan |
| author_facet | Amr T. M. Saeb Ahmad S. Alshammari Hessa Al-Brahim Khalid A. Al-Rubeaan |
| author_sort | Amr T. M. Saeb |
| collection | DOAJ |
| description | Aims. To synthesize, characterize, and analyze antimicrobial activity of AgNPs of Escherichia hermannii (SHE), Citrobacter sedlakii (S11P), and Pseudomonas putida (S5). Methods. The synthesized AgNPs were examined using ultraviolet-visible spectroscopy (UV-vis) and, zeta potential, and the size and the morphology obtained from the three different isolates were also confirmed by TEM. Results. Among the three isolates tested, SHE showed the best antimicrobial activity due to the presence of small (4–12 nm) and stable (−22 mV) AgNPs. Stability of AgNPs was also investigated and found to be dependent on the nature of isolates. Conclusion. Produced AgNPs showed particle stability and antimicrobial efficacy up to 90 days of production. Our AgNPs exhibited greater antimicrobial activity compared with gentamicin against P. aeruginosa isolates and vancomycin against S. aureus and MRSA isolates at very low concentration (0.0002 mg per Microliters). |
| format | Article |
| id | doaj-art-c1d081653d1640d8909b6dca6bcc61b4 |
| institution | DOAJ |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-c1d081653d1640d8909b6dca6bcc61b42025-08-20T03:23:27ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/704708704708Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant BacteriaAmr T. M. Saeb0Ahmad S. Alshammari1Hessa Al-Brahim2Khalid A. Al-Rubeaan3Strategic Center for Diabetes Research, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi ArabiaThe National Nanotechnology Center, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi ArabiaStrategic Center for Diabetes Research, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi ArabiaStrategic Center for Diabetes Research, King Saud University, P.O. Box 18397, Riyadh 11415, Saudi ArabiaAims. To synthesize, characterize, and analyze antimicrobial activity of AgNPs of Escherichia hermannii (SHE), Citrobacter sedlakii (S11P), and Pseudomonas putida (S5). Methods. The synthesized AgNPs were examined using ultraviolet-visible spectroscopy (UV-vis) and, zeta potential, and the size and the morphology obtained from the three different isolates were also confirmed by TEM. Results. Among the three isolates tested, SHE showed the best antimicrobial activity due to the presence of small (4–12 nm) and stable (−22 mV) AgNPs. Stability of AgNPs was also investigated and found to be dependent on the nature of isolates. Conclusion. Produced AgNPs showed particle stability and antimicrobial efficacy up to 90 days of production. Our AgNPs exhibited greater antimicrobial activity compared with gentamicin against P. aeruginosa isolates and vancomycin against S. aureus and MRSA isolates at very low concentration (0.0002 mg per Microliters).http://dx.doi.org/10.1155/2014/704708 |
| spellingShingle | Amr T. M. Saeb Ahmad S. Alshammari Hessa Al-Brahim Khalid A. Al-Rubeaan Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria The Scientific World Journal |
| title | Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria |
| title_full | Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria |
| title_fullStr | Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria |
| title_full_unstemmed | Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria |
| title_short | Production of Silver Nanoparticles with Strong and Stable Antimicrobial Activity against Highly Pathogenic and Multidrug Resistant Bacteria |
| title_sort | production of silver nanoparticles with strong and stable antimicrobial activity against highly pathogenic and multidrug resistant bacteria |
| url | http://dx.doi.org/10.1155/2014/704708 |
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