Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate

Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate

Summary: Here, we present a protocol for a glycolytic stress test using HYlight, a fluorescent protein-based biosensor that specifically binds fructose 1,6-bisphosphate (FBP), a product of the rate-limiting step of glycolysis. We describe steps for HYlight delivery into cells, real-time imaging by c...

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Bibliographic Details
Main Authors: Israel Pérez-Chávez, Nicolas Baeyens, Daria Ezeriņa, Esteban N. Gurzov, Joris Messens
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Single cell
Metabolism
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001984
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