Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood
In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the corresponding CD34pos cells, suggesting that MNC may also contain CD34neg HPC. To clarify the phenotype of AB HPC which generate EBs in these cultures, flow cytomet...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/602483 |
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| author | Valentina Tirelli Barbara Ghinassi Anna Rita Migliaccio Carolyn Whitsett Francesca Masiello Massimo Sanchez Giovanni Migliaccio |
| author_facet | Valentina Tirelli Barbara Ghinassi Anna Rita Migliaccio Carolyn Whitsett Francesca Masiello Massimo Sanchez Giovanni Migliaccio |
| author_sort | Valentina Tirelli |
| collection | DOAJ |
| description | In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the corresponding CD34pos cells, suggesting that MNC may also contain CD34neg HPC. To clarify the phenotype of AB HPC which generate EBs in these cultures, flow cytometric profiling for CD34/CD36 expression, followed by isolation and functional characterization (colony-forming-ability in semisolid-media and fold-increase in HEMA) were performed. Four populations with erythroid differentiation potential were identified: CD34posCD36neg (0.1%); (barely detectable-0.1%); (2%) and (75%). In semisolid-media, cells generated BFU-E and CFU-GM (in a 1 : 1 ratio), cells mostly BFU-E (87%) and and CD34negCD36low cells were not tested due to low numbers. Under HEMA conditions, CD34posCD36neg, CD34posCD36pos, CD34negCD36low and CD34negCD36neg cells generated EBs with fold-increases of ≈9,000, 100, 60 and 1, respectively, and maturation times (day with >10% CD36highCD235ahigh cells) of 10–7 days. Pyrenocytes were generated only by CD34neg/CD36neg cells by day 15. These results confirm that the majority of HPC in AB express CD34 but identify additional CD34neg populations with erythroid differentiation potential which, based on differences in fold-increase and maturation times, may represent a hierarchy of HPC present in AB. |
| format | Article |
| id | doaj-art-c19d3f346e7d4b5abc63c8a1e922798d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c19d3f346e7d4b5abc63c8a1e922798d2025-02-03T06:44:47ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/602483602483Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult BloodValentina Tirelli0Barbara Ghinassi1Anna Rita Migliaccio2Carolyn Whitsett3Francesca Masiello4Massimo Sanchez5Giovanni Migliaccio6Cell Biology and Neuroscience, Superior Health Institute, 00161 Rome, ItalyTisch Cancer Institute, Mount Sinai School of Medicine, New York, NY 10029, USATisch Cancer Institute, Mount Sinai School of Medicine, New York, NY 10029, USATisch Cancer Institute, Mount Sinai School of Medicine, New York, NY 10029, USACell Biology and Neuroscience, Superior Health Institute, 00161 Rome, ItalyCell Biology and Neuroscience, Superior Health Institute, 00161 Rome, ItalyCell Biology and Neuroscience, Superior Health Institute, 00161 Rome, ItalyIn Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the corresponding CD34pos cells, suggesting that MNC may also contain CD34neg HPC. To clarify the phenotype of AB HPC which generate EBs in these cultures, flow cytometric profiling for CD34/CD36 expression, followed by isolation and functional characterization (colony-forming-ability in semisolid-media and fold-increase in HEMA) were performed. Four populations with erythroid differentiation potential were identified: CD34posCD36neg (0.1%); (barely detectable-0.1%); (2%) and (75%). In semisolid-media, cells generated BFU-E and CFU-GM (in a 1 : 1 ratio), cells mostly BFU-E (87%) and and CD34negCD36low cells were not tested due to low numbers. Under HEMA conditions, CD34posCD36neg, CD34posCD36pos, CD34negCD36low and CD34negCD36neg cells generated EBs with fold-increases of ≈9,000, 100, 60 and 1, respectively, and maturation times (day with >10% CD36highCD235ahigh cells) of 10–7 days. Pyrenocytes were generated only by CD34neg/CD36neg cells by day 15. These results confirm that the majority of HPC in AB express CD34 but identify additional CD34neg populations with erythroid differentiation potential which, based on differences in fold-increase and maturation times, may represent a hierarchy of HPC present in AB.http://dx.doi.org/10.4061/2011/602483 |
| spellingShingle | Valentina Tirelli Barbara Ghinassi Anna Rita Migliaccio Carolyn Whitsett Francesca Masiello Massimo Sanchez Giovanni Migliaccio Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood Stem Cells International |
| title | Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood |
| title_full | Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood |
| title_fullStr | Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood |
| title_full_unstemmed | Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood |
| title_short | Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood |
| title_sort | phenotypic definition of the progenitor cells with erythroid differentiation potential present in human adult blood |
| url | http://dx.doi.org/10.4061/2011/602483 |
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