Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva
Biosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting SARS-CoV-2. Unlike conventional methods requiri...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/25/2/360 |
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| author | Paweł Wityk Agata Terebieniec Robert Nowak Jacek Łubiński Martyna Mroczyńska-Szeląg Tomasz Wityk Dorota Kostrzewa-Nowak |
| author_facet | Paweł Wityk Agata Terebieniec Robert Nowak Jacek Łubiński Martyna Mroczyńska-Szeląg Tomasz Wityk Dorota Kostrzewa-Nowak |
| author_sort | Paweł Wityk |
| collection | DOAJ |
| description | Biosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting SARS-CoV-2. Unlike conventional methods requiring filtration, our biosensor leverages a unique electrode design that prevents interference from saliva debris, allowing precise measurements. The biosensor is based on electrochemical principles, employing oligonucleotide probes immobilized on a hydrophobic-coated electrode, which prevents air bubbles and salt crystal formation. During validation, the biosensor demonstrated a sensitivity and specificity of 100%, accurately identifying SARS-CoV-2 in saliva samples without false positives or negatives. Cross-validation with RT-qPCR, the gold standard for COVID-19 diagnostics, confirmed the reliability of our device. The biosensor’s performance was tested on 60 participants, yielding 12 true positive results and 48 true negatives, aligning perfectly with RT-qPCR outcomes. This reusable, easy-to-use biosensor offers significant potential for point-of-care applications in various healthcare settings, providing a fast, efficient, and cost-effective method for detecting viral infections such as COVID-19. Its robust design, minimal sample preparation requirements, and multiple-use capability mark a significant advancement in biosensing technology. |
| format | Article |
| id | doaj-art-c18b0975dcfc42bcaa9b08a18b61d743 |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-c18b0975dcfc42bcaa9b08a18b61d7432025-01-24T13:48:38ZengMDPI AGSensors1424-82202025-01-0125236010.3390/s25020360Reusable Biosensor for Easy RNA Detection from Unfiltered SalivaPaweł Wityk0Agata Terebieniec1Robert Nowak2Jacek Łubiński3Martyna Mroczyńska-Szeląg4Tomasz Wityk5Dorota Kostrzewa-Nowak6Map Your DNA Ltd., Świerkowa 40, 83-330 Lniska, PolandFungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, 3584 CS Utrecht, The NetherlandsInstitute of Physical Culture Sciences, University of Szczecin, 40B Piastów Al., Building 6, 71-065 Szczecin, PolandFaculty of Mechanical Engineering and Ship Technology, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, PolandFaculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, PolandMap Your DNA Ltd., Świerkowa 40, 83-330 Lniska, PolandDepartment of Clinical and Molecular Biochemistry, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp. Al., 70-111 Szczecin, PolandBiosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting SARS-CoV-2. Unlike conventional methods requiring filtration, our biosensor leverages a unique electrode design that prevents interference from saliva debris, allowing precise measurements. The biosensor is based on electrochemical principles, employing oligonucleotide probes immobilized on a hydrophobic-coated electrode, which prevents air bubbles and salt crystal formation. During validation, the biosensor demonstrated a sensitivity and specificity of 100%, accurately identifying SARS-CoV-2 in saliva samples without false positives or negatives. Cross-validation with RT-qPCR, the gold standard for COVID-19 diagnostics, confirmed the reliability of our device. The biosensor’s performance was tested on 60 participants, yielding 12 true positive results and 48 true negatives, aligning perfectly with RT-qPCR outcomes. This reusable, easy-to-use biosensor offers significant potential for point-of-care applications in various healthcare settings, providing a fast, efficient, and cost-effective method for detecting viral infections such as COVID-19. Its robust design, minimal sample preparation requirements, and multiple-use capability mark a significant advancement in biosensing technology.https://www.mdpi.com/1424-8220/25/2/360biosensorcapacitanceinfectionsalivaEIS |
| spellingShingle | Paweł Wityk Agata Terebieniec Robert Nowak Jacek Łubiński Martyna Mroczyńska-Szeląg Tomasz Wityk Dorota Kostrzewa-Nowak Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva Sensors biosensor capacitance infection saliva EIS |
| title | Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva |
| title_full | Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva |
| title_fullStr | Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva |
| title_full_unstemmed | Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva |
| title_short | Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva |
| title_sort | reusable biosensor for easy rna detection from unfiltered saliva |
| topic | biosensor capacitance infection saliva EIS |
| url | https://www.mdpi.com/1424-8220/25/2/360 |
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