Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acid
Optimal extraction parameters were determined, yielding 12.4 % oil at 4.5 h, with a particle size of 1.375 mm and a solute-to-solvent ratio of 0.28 w/v. The extracted oil exhibited high yield (15 % moisture, 0.92 g/mL specific gravity), a volatile lubricant component (3 %), a pH of 4.2–5.91, low ash...
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Elsevier
2025-01-01
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| author | Dereje Ayele Gebreyes Melakuu Tesfaye Alemea Alemu Gonfa Robi Workiye Getnet Abera Getachew Alemu Tenkolu |
| author_facet | Dereje Ayele Gebreyes Melakuu Tesfaye Alemea Alemu Gonfa Robi Workiye Getnet Abera Getachew Alemu Tenkolu |
| author_sort | Dereje Ayele Gebreyes |
| collection | DOAJ |
| description | Optimal extraction parameters were determined, yielding 12.4 % oil at 4.5 h, with a particle size of 1.375 mm and a solute-to-solvent ratio of 0.28 w/v. The extracted oil exhibited high yield (15 % moisture, 0.92 g/mL specific gravity), a volatile lubricant component (3 %), a pH of 4.2–5.91, low ash (11.5 %), high saponification value (19.7 g KOH/g) and moderate acid number (8.9 mg KOH/g), iodine value of 35.3 g/L, low viscosity (0.1160 Pa s), and high diffusion capacity (95 %). Chemical analysis using UV–Vis spectrophotometry was employed to identify the constituents present in the extract, while FTIR spectra indicated the presence of alcoholic and phenolic components, suggesting interactions with lactic acid. Through GC/MS analysis, a total of 32 phytochemicals were identified, with seven significant ones: Eugenol (Phenol), Isocaryophyllene, Elemicin (benzene), Hexadecanoic acid with methyl ester, Octadecanoic acid with methyl ester, Neophytadiene and Squalene. Evaluation against E. coli, B. subtilis, S. typhimurium, and S. aureus revealed the O. lamiifolium extract and biolubricants most active against S. aureus and E. coli, suggesting potential for superior antibacterial lubricants with this plant extract and lactic acid. |
| format | Article |
| id | doaj-art-c12d6ce4338c44aab9b66ba18e33a9c2 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-c12d6ce4338c44aab9b66ba18e33a9c22025-02-02T05:27:45ZengElsevierHeliyon2405-84402025-01-01112e38972Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acidDereje Ayele Gebreyes0Melakuu Tesfaye Alemea1Alemu Gonfa Robi2Workiye Getnet Abera3Getachew Alemu Tenkolu4Department of Chemical Engineering, Adama Science and Technology University, Adama, Ethiopia; Department of Chemical and Food Engineering, College of Engineering, Dilla University, Dilla, EthiopiaDepartment of Chemical Engineering, Adama Science and Technology University, Adama, Ethiopia; Corresponding author.Department of Chemical Engineering, Adama Science and Technology University, Adama, EthiopiaDepartment of Chemical and Food Engineering, College of Engineering, Dilla University, Dilla, Ethiopia; Department of Chemical Engineering, Gafat Institute of Technology, Debre Tabor University, Debre Tabor, Ethiopia; Corresponding author. Department of Chemical and Food Engineering, College of Engineering, Dilla University, Dilla, Ethiopia.Department of Chemical Engineering, Institute of Technology, Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia; School of Energy and Power Engineering, Jiangsu University, Zhenjiang, 212013, ChinaOptimal extraction parameters were determined, yielding 12.4 % oil at 4.5 h, with a particle size of 1.375 mm and a solute-to-solvent ratio of 0.28 w/v. The extracted oil exhibited high yield (15 % moisture, 0.92 g/mL specific gravity), a volatile lubricant component (3 %), a pH of 4.2–5.91, low ash (11.5 %), high saponification value (19.7 g KOH/g) and moderate acid number (8.9 mg KOH/g), iodine value of 35.3 g/L, low viscosity (0.1160 Pa s), and high diffusion capacity (95 %). Chemical analysis using UV–Vis spectrophotometry was employed to identify the constituents present in the extract, while FTIR spectra indicated the presence of alcoholic and phenolic components, suggesting interactions with lactic acid. Through GC/MS analysis, a total of 32 phytochemicals were identified, with seven significant ones: Eugenol (Phenol), Isocaryophyllene, Elemicin (benzene), Hexadecanoic acid with methyl ester, Octadecanoic acid with methyl ester, Neophytadiene and Squalene. Evaluation against E. coli, B. subtilis, S. typhimurium, and S. aureus revealed the O. lamiifolium extract and biolubricants most active against S. aureus and E. coli, suggesting potential for superior antibacterial lubricants with this plant extract and lactic acid.http://www.sciencedirect.com/science/article/pii/S2405844024150037Bio-lubricantFTIRGC-MSOcimum lamiifoliumRSM box-behnkenSoxhlet extraction |
| spellingShingle | Dereje Ayele Gebreyes Melakuu Tesfaye Alemea Alemu Gonfa Robi Workiye Getnet Abera Getachew Alemu Tenkolu Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acid Heliyon Bio-lubricant FTIR GC-MS Ocimum lamiifolium RSM box-behnken Soxhlet extraction |
| title | Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acid |
| title_full | Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acid |
| title_fullStr | Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acid |
| title_full_unstemmed | Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acid |
| title_short | Synthesis, characterization, and optimization of antimicrobial biolubricant derived from Ocimum lamiifolium and lactic acid |
| title_sort | synthesis characterization and optimization of antimicrobial biolubricant derived from ocimum lamiifolium and lactic acid |
| topic | Bio-lubricant FTIR GC-MS Ocimum lamiifolium RSM box-behnken Soxhlet extraction |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024150037 |
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