Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2015/289510 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547036527853568 |
|---|---|
| author | Marina Okuyama Kishima Karen Brajão de Oliveira Carolina Batista Ariza Carlos Eduardo Coral de Oliveira Roberta Losi Guembarovski Bruna Karina Banin Hirata Felipe Campos de Almeida Glauco Akelinghton Freire Vitiello Kleber Paiva Trugilo Alda Fiorina Maria Losi Guembarovski Walter Jorge Sobrinho Clodoaldo Zago Campos Maria Angelica Ehara Watanabe |
| author_facet | Marina Okuyama Kishima Karen Brajão de Oliveira Carolina Batista Ariza Carlos Eduardo Coral de Oliveira Roberta Losi Guembarovski Bruna Karina Banin Hirata Felipe Campos de Almeida Glauco Akelinghton Freire Vitiello Kleber Paiva Trugilo Alda Fiorina Maria Losi Guembarovski Walter Jorge Sobrinho Clodoaldo Zago Campos Maria Angelica Ehara Watanabe |
| author_sort | Marina Okuyama Kishima |
| collection | DOAJ |
| description | CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients presented higher CXCR4 mRNA relative expression (5.7-fold) than normal mammary gland, but this expression was not correlated with patients clinicopathological features (nuclear grade, nodal status, ER status, PR status, p53 staining, Ki67 index, and HER-2 status). Moreover, CXCR4 mRNA relative expression also did not differ regarding the presence or absence of T allele (p=0.301). In the immunohistochemical assay, no difference was observed for CXCR4 cytoplasmic protein staining in relation to different genotypes (p=0.757); however, high cytoplasmic CXCR4 staining was verified in invasive breast carcinoma (p<0.01). All in all, the results from present study indicated that rs2228014 genetic variant does not alter CXCR4 mRNA or protein expression. However, this receptor was more expressed in tumor compared to normal tissue, in both RNA and protein levels, suggesting its promising applicability in the general context of mammary carcinogenesis. |
| format | Article |
| id | doaj-art-c117689b57de4a00b80f2d135bce9e34 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-c117689b57de4a00b80f2d135bce9e342025-02-03T06:46:17ZengWileyAnalytical Cellular Pathology2210-71772210-71852015-01-01201510.1155/2015/289510289510Genetic Polymorphism and Expression of CXCR4 in Breast CancerMarina Okuyama Kishima0Karen Brajão de Oliveira1Carolina Batista Ariza2Carlos Eduardo Coral de Oliveira3Roberta Losi Guembarovski4Bruna Karina Banin Hirata5Felipe Campos de Almeida6Glauco Akelinghton Freire Vitiello7Kleber Paiva Trugilo8Alda Fiorina Maria Losi Guembarovski9Walter Jorge Sobrinho10Clodoaldo Zago Campos11Maria Angelica Ehara Watanabe12Laboratory of Human Pathology, Department of Pathology, Clinical Analysis and Toxicology, State University of Londrina, Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilLaboratory of Human Pathology, Department of Pathology, Clinical Analysis and Toxicology, State University of Londrina, Londrina, PR, BrazilGineco-Med Clinical of Mastology, Londrina, PR, BrazilCancer Hospital of Londrina, Londrina, PR, BrazilDepartment of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, BrazilCXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients presented higher CXCR4 mRNA relative expression (5.7-fold) than normal mammary gland, but this expression was not correlated with patients clinicopathological features (nuclear grade, nodal status, ER status, PR status, p53 staining, Ki67 index, and HER-2 status). Moreover, CXCR4 mRNA relative expression also did not differ regarding the presence or absence of T allele (p=0.301). In the immunohistochemical assay, no difference was observed for CXCR4 cytoplasmic protein staining in relation to different genotypes (p=0.757); however, high cytoplasmic CXCR4 staining was verified in invasive breast carcinoma (p<0.01). All in all, the results from present study indicated that rs2228014 genetic variant does not alter CXCR4 mRNA or protein expression. However, this receptor was more expressed in tumor compared to normal tissue, in both RNA and protein levels, suggesting its promising applicability in the general context of mammary carcinogenesis.http://dx.doi.org/10.1155/2015/289510 |
| spellingShingle | Marina Okuyama Kishima Karen Brajão de Oliveira Carolina Batista Ariza Carlos Eduardo Coral de Oliveira Roberta Losi Guembarovski Bruna Karina Banin Hirata Felipe Campos de Almeida Glauco Akelinghton Freire Vitiello Kleber Paiva Trugilo Alda Fiorina Maria Losi Guembarovski Walter Jorge Sobrinho Clodoaldo Zago Campos Maria Angelica Ehara Watanabe Genetic Polymorphism and Expression of CXCR4 in Breast Cancer Analytical Cellular Pathology |
| title | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
| title_full | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
| title_fullStr | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
| title_full_unstemmed | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
| title_short | Genetic Polymorphism and Expression of CXCR4 in Breast Cancer |
| title_sort | genetic polymorphism and expression of cxcr4 in breast cancer |
| url | http://dx.doi.org/10.1155/2015/289510 |
| work_keys_str_mv | AT marinaokuyamakishima geneticpolymorphismandexpressionofcxcr4inbreastcancer AT karenbrajaodeoliveira geneticpolymorphismandexpressionofcxcr4inbreastcancer AT carolinabatistaariza geneticpolymorphismandexpressionofcxcr4inbreastcancer AT carloseduardocoraldeoliveira geneticpolymorphismandexpressionofcxcr4inbreastcancer AT robertalosiguembarovski geneticpolymorphismandexpressionofcxcr4inbreastcancer AT brunakarinabaninhirata geneticpolymorphismandexpressionofcxcr4inbreastcancer AT felipecamposdealmeida geneticpolymorphismandexpressionofcxcr4inbreastcancer AT glaucoakelinghtonfreirevitiello geneticpolymorphismandexpressionofcxcr4inbreastcancer AT kleberpaivatrugilo geneticpolymorphismandexpressionofcxcr4inbreastcancer AT aldafiorinamarialosiguembarovski geneticpolymorphismandexpressionofcxcr4inbreastcancer AT walterjorgesobrinho geneticpolymorphismandexpressionofcxcr4inbreastcancer AT clodoaldozagocampos geneticpolymorphismandexpressionofcxcr4inbreastcancer AT mariaangelicaeharawatanabe geneticpolymorphismandexpressionofcxcr4inbreastcancer |