Advances in the detection of virulence genes of Staphylococcus aureus originate from food
Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it is necessary to detect pathogenic bacteria. The virulence genes and detection methods of S. aureus are summarized from...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2020-03-01
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| Series: | Food Science and Human Wellness |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453019302630 |
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| author | Yingying Zhao Dandan Xia Panpan Ma Xiaofeng Gao Wenyi Kang Jinfeng Wei |
| author_facet | Yingying Zhao Dandan Xia Panpan Ma Xiaofeng Gao Wenyi Kang Jinfeng Wei |
| author_sort | Yingying Zhao |
| collection | DOAJ |
| description | Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it is necessary to detect pathogenic bacteria. The virulence genes and detection methods of S. aureus are summarized from literatures. Traditional detection methods are simple but need bacteria enrichment and to prolong detection time. Immunological technology has high specificity, but false positive results are easy to occur in its detection. In recent years, molecular biology methods have developed rapidly, and various PCR techniques have been applied to detect foodborne pathogens. Real-time fluorescent quantitative PCR is more effective than ordinary PCR, while price is expensive when use it, the scope of use is limited. LAMP is a flexible detection method, biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive. Keywords: Staphylococcus aureus, Virulence genes, Conventional methods, Rapid detection methods |
| format | Article |
| id | doaj-art-c073a44882474a8da40b943eb93cde72 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c073a44882474a8da40b943eb93cde722025-02-03T06:14:24ZengTsinghua University PressFood Science and Human Wellness2213-45302020-03-01914044Advances in the detection of virulence genes of Staphylococcus aureus originate from foodYingying Zhao0Dandan Xia1Panpan Ma2Xiaofeng Gao3Wenyi Kang4Jinfeng Wei5National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China; Minsheng College, Henan University, Kaifeng, Henan 475004, China; Corresponding author.Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it is necessary to detect pathogenic bacteria. The virulence genes and detection methods of S. aureus are summarized from literatures. Traditional detection methods are simple but need bacteria enrichment and to prolong detection time. Immunological technology has high specificity, but false positive results are easy to occur in its detection. In recent years, molecular biology methods have developed rapidly, and various PCR techniques have been applied to detect foodborne pathogens. Real-time fluorescent quantitative PCR is more effective than ordinary PCR, while price is expensive when use it, the scope of use is limited. LAMP is a flexible detection method, biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive. Keywords: Staphylococcus aureus, Virulence genes, Conventional methods, Rapid detection methodshttp://www.sciencedirect.com/science/article/pii/S2213453019302630 |
| spellingShingle | Yingying Zhao Dandan Xia Panpan Ma Xiaofeng Gao Wenyi Kang Jinfeng Wei Advances in the detection of virulence genes of Staphylococcus aureus originate from food Food Science and Human Wellness |
| title | Advances in the detection of virulence genes of Staphylococcus aureus originate from food |
| title_full | Advances in the detection of virulence genes of Staphylococcus aureus originate from food |
| title_fullStr | Advances in the detection of virulence genes of Staphylococcus aureus originate from food |
| title_full_unstemmed | Advances in the detection of virulence genes of Staphylococcus aureus originate from food |
| title_short | Advances in the detection of virulence genes of Staphylococcus aureus originate from food |
| title_sort | advances in the detection of virulence genes of staphylococcus aureus originate from food |
| url | http://www.sciencedirect.com/science/article/pii/S2213453019302630 |
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