Potential influence of the first PCR cycles in real-time comparative gene quantifications
There is an underlying assumption in real-time PCR that the amplification efficiency is equal from the first cycles until a signal can be detected. In this study, we evaluated this assumption by analyzing genes with known gene copy number using real-time PCR comparative gene quantifications. Listeri...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04372RR01 |
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