In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex Baker
The corms of Hypoxis argentea are widely used as a traditional remedy for diabetes mellitus in South Africa. In this study, we investigated the effects of non-toxic concentrations (12.5-100 μg mL-1) of the aqueous extract of H. argentea (HAA) corms on glucose uptake, pancreatic beta cell proliferati...
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| Format: | Article |
| Language: | English |
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2018-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2018-0023 |
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| author | Akinrinde Akinleye Koekemoer Trevor Venter Maryna Van De Bradley Graeme |
| author_facet | Akinrinde Akinleye Koekemoer Trevor Venter Maryna Van De Bradley Graeme |
| author_sort | Akinrinde Akinleye |
| collection | DOAJ |
| description | The corms of Hypoxis argentea are widely used as a traditional remedy for diabetes mellitus in South Africa. In this study, we investigated the effects of non-toxic concentrations (12.5-100 μg mL-1) of the aqueous extract of H. argentea (HAA) corms on glucose uptake, pancreatic beta cell proliferation, and adipocyte differentiation. HAA stimulated glucose uptake in HepG2 cells up to 19.6 % and 17.0 % in L6 myotubes. Live-cell imaging microscopy revealed significant increases (p < 0.001) in total INS-1 cell numbers exposed to HAA, although no effect was observed on adipogenesis in 3T3-L1 pre-adipocytes. HAA produced weak to moderate inhibition of porcine pancreatic α-amylase, α-glucosidase, porcine pancreatic lipase, dipeptidyl peptidase IV (DPP IV) activities, as well as protein glycation. Our results suggest that the acclaimed anti-diabetic effects of H. argentea could be mediated by its promotion of glucose utilization and preservation of pancreatic beta cell populations while preventing fat accumulation in adipocytes. |
| format | Article |
| id | doaj-art-befd25e185aa4501bd3b2055ebe8daa9 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-befd25e185aa4501bd3b2055ebe8daa92025-02-02T11:05:46ZengSciendoActa Pharmaceutica1846-95582018-12-0168438940710.2478/acph-2018-0023acph-2018-0023In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex BakerAkinrinde Akinleye0Koekemoer Trevor1Venter Maryna Van De2Bradley Graeme3Plant stress response group Department of Biochemistry and Microbiology, University of Fort Hare Alice, 5700,Alice, South AfricaMedicinal Plants & Natural Products Research Group, Department of Biochemistry and Microbiology Nelson Mandela Metropolitan University,Port ElizabethSouth AfricaMedicinal Plants & Natural Products Research Group, Department of Biochemistry and Microbiology Nelson Mandela Metropolitan University,Port ElizabethSouth AfricaPlant stress response group Department of Biochemistry and Microbiology, University of Fort Hare Alice, 5700,Alice, South AfricaThe corms of Hypoxis argentea are widely used as a traditional remedy for diabetes mellitus in South Africa. In this study, we investigated the effects of non-toxic concentrations (12.5-100 μg mL-1) of the aqueous extract of H. argentea (HAA) corms on glucose uptake, pancreatic beta cell proliferation, and adipocyte differentiation. HAA stimulated glucose uptake in HepG2 cells up to 19.6 % and 17.0 % in L6 myotubes. Live-cell imaging microscopy revealed significant increases (p < 0.001) in total INS-1 cell numbers exposed to HAA, although no effect was observed on adipogenesis in 3T3-L1 pre-adipocytes. HAA produced weak to moderate inhibition of porcine pancreatic α-amylase, α-glucosidase, porcine pancreatic lipase, dipeptidyl peptidase IV (DPP IV) activities, as well as protein glycation. Our results suggest that the acclaimed anti-diabetic effects of H. argentea could be mediated by its promotion of glucose utilization and preservation of pancreatic beta cell populations while preventing fat accumulation in adipocytes.https://doi.org/10.2478/acph-2018-0023hypoxis argenteadiabeteshepg2ins-13t3-l1l6 myotubesglucose uptakeadipogenesis |
| spellingShingle | Akinrinde Akinleye Koekemoer Trevor Venter Maryna Van De Bradley Graeme In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex Baker Acta Pharmaceutica hypoxis argentea diabetes hepg2 ins-1 3t3-l1 l6 myotubes glucose uptake adipogenesis |
| title | In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex Baker |
| title_full | In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex Baker |
| title_fullStr | In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex Baker |
| title_full_unstemmed | In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex Baker |
| title_short | In Vitro Investigation of Potential Anti-Diabetic Activity of the Corm Extract of Hypoxis Argentea Harv. Ex Baker |
| title_sort | in vitro investigation of potential anti diabetic activity of the corm extract of hypoxis argentea harv ex baker |
| topic | hypoxis argentea diabetes hepg2 ins-1 3t3-l1 l6 myotubes glucose uptake adipogenesis |
| url | https://doi.org/10.2478/acph-2018-0023 |
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