鬼臼乙叉苷对人鼻咽癌细胞CNE2的细胞毒作用和对DNA的断裂作用
作者用噻唑蓝还原（MTT）法测定了鬼臼乙叉苷（鬼臼乙叉甙,VP-16）对人体鼻咽癌细胞CNE2的毒性作用,在药物浓度为2.5,5.0,10.0,20.0,40.0,80.0和160.0 ug/ml的条件下,连续4次实验,VP-16对CNE2细胞的生长抑制率分别为3.3±3.7％,15.2±3％,36.l±l0％,54±17％,68.4％士13％,76.7士9％和84.2±8％,其半数抑制浓度(IC50)为17.0 ug/ml。CNE2细胞分别在含有20.0,100.0和200.0ug/ml VP-16的培养液中孵育24h,细胞的DNA发生了断裂。DNA断裂量随药物浓度的增加而增加。将CNE2细...
Saved in:
| Format: | Article |
|---|---|
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
1993-01-01
|
| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/44124284/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|