Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana
Abstract RNA methylation plays a central regulatory role in plant biology and is a relatively new target for plant improvement efforts. In nearly all cases, perturbation of the RNA methylation machinery results in deleterious phenotypes. However, a recent landmark paper reported that transcriptome‐w...
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| Format: | Article |
| Language: | English |
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Wiley
2024-08-01
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| Series: | Plant Direct |
| Online Access: | https://doi.org/10.1002/pld3.70000 |
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| _version_ | 1832087824990470144 |
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| author | Kasey Markel Lucas Waldburger Patrick M. Shih |
| author_facet | Kasey Markel Lucas Waldburger Patrick M. Shih |
| author_sort | Kasey Markel |
| collection | DOAJ |
| description | Abstract RNA methylation plays a central regulatory role in plant biology and is a relatively new target for plant improvement efforts. In nearly all cases, perturbation of the RNA methylation machinery results in deleterious phenotypes. However, a recent landmark paper reported that transcriptome‐wide use of the human RNA demethylase FTO substantially increased the yield of rice and potatoes. Here, we have performed the first independent replication of those results and demonstrated broader transferability of the trait, finding increased flower and fruit count in the model species Arabidopsis thaliana. We also performed RNA‐seq of our FTO‐transgenic plants, which we analyzed in conjunction with previously published datasets to detect several previously unrecognized patterns in the functional and structural classification of the upregulated and downregulated genes. From these, we present mechanistic hypotheses to explain these surprising results with the goal of spurring more widespread interest in this promising new approach to plant engineering. |
| format | Article |
| id | doaj-art-be5c411bc83c457e8b17441eb19d7a2c |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-be5c411bc83c457e8b17441eb19d7a2c2025-02-06T04:40:50ZengWileyPlant Direct2475-44552024-08-0188n/an/a10.1002/pld3.70000Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thalianaKasey Markel0Lucas Waldburger1Patrick M. Shih2Department of Plant and Microbial Biology University of California Berkeley California USAEnvironmental Genomics and Systems Biology Division Lawrence Berkeley National Laboratory Berkeley California USADepartment of Plant and Microbial Biology University of California Berkeley California USAAbstract RNA methylation plays a central regulatory role in plant biology and is a relatively new target for plant improvement efforts. In nearly all cases, perturbation of the RNA methylation machinery results in deleterious phenotypes. However, a recent landmark paper reported that transcriptome‐wide use of the human RNA demethylase FTO substantially increased the yield of rice and potatoes. Here, we have performed the first independent replication of those results and demonstrated broader transferability of the trait, finding increased flower and fruit count in the model species Arabidopsis thaliana. We also performed RNA‐seq of our FTO‐transgenic plants, which we analyzed in conjunction with previously published datasets to detect several previously unrecognized patterns in the functional and structural classification of the upregulated and downregulated genes. From these, we present mechanistic hypotheses to explain these surprising results with the goal of spurring more widespread interest in this promising new approach to plant engineering.https://doi.org/10.1002/pld3.70000 |
| spellingShingle | Kasey Markel Lucas Waldburger Patrick M. Shih Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana Plant Direct |
| title | Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana |
| title_full | Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana |
| title_fullStr | Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana |
| title_full_unstemmed | Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana |
| title_short | Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana |
| title_sort | expression of a mammalian rna demethylase increases flower number and floral stem branching in arabidopsis thaliana |
| url | https://doi.org/10.1002/pld3.70000 |
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