Measuring plant cysteine oxidase interactions with substrates using intrinsic tryptophan fluorescence

Abstract Plant Cysteine Oxidases (PCOs) are oxygen-sensing enyzmes that catalyse oxidation of cysteinyl residues at the N-termini of target proteins, triggering their degradation via the N-degron pathway. PCO oxygen sensitivity means that in low oxygen conditions (hypoxia), their activity reduces an...

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Bibliographic Details
Main Authors: Dona M. Gunawardana, Daisy A. Southern, Emily Flashman
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-024-83508-y
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