Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor
In this study, sol gel technique is used to fabricate manganese (Mn) doped ZnO thin films and further utilize them as a platform for uric acid biosensors. The objective was to introduce manganese into the ZnO matrix to enhance its redox properties, capitalizing on the multivalent nature of manganese...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Chemical Physics Impact |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667022425000118 |
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| author | Bilasini Devi Naorem Jatinder Pal Singh Babita Sharma Satyam Garg Athira C Hashima Sherin Mahima Momaliya Muskan Shubhi Sahu Arijit Chowdhuri Mallika Verma Monika Tomar Neha Batra |
| author_facet | Bilasini Devi Naorem Jatinder Pal Singh Babita Sharma Satyam Garg Athira C Hashima Sherin Mahima Momaliya Muskan Shubhi Sahu Arijit Chowdhuri Mallika Verma Monika Tomar Neha Batra |
| author_sort | Bilasini Devi Naorem |
| collection | DOAJ |
| description | In this study, sol gel technique is used to fabricate manganese (Mn) doped ZnO thin films and further utilize them as a platform for uric acid biosensors. The objective was to introduce manganese into the ZnO matrix to enhance its redox properties, capitalizing on the multivalent nature of manganese. The Mn-doped thin films of concentrations varying from 3 %,5 %,7 % and 10 % were prepared and further characterized using UV–vis spectroscopy, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM) and cyclic voltammetry (CV) measurements. The ZnO thin films with 7 % doping of Mn exhibited improved redox behaviour, as evident by the distinct redox peaks. In order to immobilise the uricase enzyme, the 7 % Mn doped composition was used, creating a highly sensitive and focused uric acid detection platform. The fabricated biosensor exhibits excellent performance in terms of sensitivity (40 µAmM-1cm-2), selectivity with <5 % deviation found in presence of other known markers present in human sera, and shelf life >12 weeks, enabling precise and sensitive uric acid detection. This study brings to light an alternate approach in developing point of care biosensors using transition metal doped ZnO thin films. |
| format | Article |
| id | doaj-art-be1abed9caa9417da76ef0d32c583105 |
| institution | Kabale University |
| issn | 2667-0224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chemical Physics Impact |
| spelling | doaj-art-be1abed9caa9417da76ef0d32c5831052025-01-20T04:18:00ZengElsevierChemical Physics Impact2667-02242025-06-0110100823Mn-doped ZnO thin films as a platform for reagentless uric acid biosensorBilasini Devi Naorem0Jatinder Pal Singh1Babita Sharma2Satyam Garg3Athira C4Hashima Sherin5Mahima Momaliya6 Muskan7Shubhi Sahu8Arijit Chowdhuri9Mallika Verma10Monika Tomar11Neha Batra12Department of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics and Astrophysics, University of Delhi, Delhi 110 007, India; Department of Physics, ANDC, University of Delhi, Delhi 110 019, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, India; Department of Physics and Astrophysics, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, India; Department of Physics and Astrophysics, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, ANDC, University of Delhi, Delhi 110 019, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Miranda House, University of Delhi, Delhi 110 007, IndiaDepartment of Physics, Hindu College, University of Delhi, Delhi 110 007, India; Corresponding author.In this study, sol gel technique is used to fabricate manganese (Mn) doped ZnO thin films and further utilize them as a platform for uric acid biosensors. The objective was to introduce manganese into the ZnO matrix to enhance its redox properties, capitalizing on the multivalent nature of manganese. The Mn-doped thin films of concentrations varying from 3 %,5 %,7 % and 10 % were prepared and further characterized using UV–vis spectroscopy, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM) and cyclic voltammetry (CV) measurements. The ZnO thin films with 7 % doping of Mn exhibited improved redox behaviour, as evident by the distinct redox peaks. In order to immobilise the uricase enzyme, the 7 % Mn doped composition was used, creating a highly sensitive and focused uric acid detection platform. The fabricated biosensor exhibits excellent performance in terms of sensitivity (40 µAmM-1cm-2), selectivity with <5 % deviation found in presence of other known markers present in human sera, and shelf life >12 weeks, enabling precise and sensitive uric acid detection. This study brings to light an alternate approach in developing point of care biosensors using transition metal doped ZnO thin films.http://www.sciencedirect.com/science/article/pii/S2667022425000118Mediator-freeMn doped ZnOUric acidBiosensor |
| spellingShingle | Bilasini Devi Naorem Jatinder Pal Singh Babita Sharma Satyam Garg Athira C Hashima Sherin Mahima Momaliya Muskan Shubhi Sahu Arijit Chowdhuri Mallika Verma Monika Tomar Neha Batra Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor Chemical Physics Impact Mediator-free Mn doped ZnO Uric acid Biosensor |
| title | Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor |
| title_full | Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor |
| title_fullStr | Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor |
| title_full_unstemmed | Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor |
| title_short | Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor |
| title_sort | mn doped zno thin films as a platform for reagentless uric acid biosensor |
| topic | Mediator-free Mn doped ZnO Uric acid Biosensor |
| url | http://www.sciencedirect.com/science/article/pii/S2667022425000118 |
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