Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants
The very low birth weight (VLBW) infant is at great risk for marked dysbiosis of the gut microbiota. In the present study, a total of 36 VLBW infants were randomly divided into two groups, who were treated with combined probiotics and placebo, and 72 fecal specimens on days 14 and 28 of life were co...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/3682836 |
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| author | Yue-feng Li Chuan-rui Zhu Xue-lei Gong Hui-ling Li Li-kuan Xiong Ke-jian Wang Guo-Sheng Liu |
| author_facet | Yue-feng Li Chuan-rui Zhu Xue-lei Gong Hui-ling Li Li-kuan Xiong Ke-jian Wang Guo-Sheng Liu |
| author_sort | Yue-feng Li |
| collection | DOAJ |
| description | The very low birth weight (VLBW) infant is at great risk for marked dysbiosis of the gut microbiota. In the present study, a total of 36 VLBW infants were randomly divided into two groups, who were treated with combined probiotics and placebo, and 72 fecal specimens on days 14 and 28 of life were collected from them. Finally, 32 fecal specimens extracted from 16 preterm VLBW infants were qualified and analyzed using 16S rRNA gene sequencing. The primary outcome was to evaluate the change of gut microbiota in VLBW infants after combined probiotic supplement. The secondary outcome was to analyze the correlation gut microbial composition and levels of cytokines. We found that probiotic treatment, but not placebo, decreased the α-diversity of gut microbiota in VLBW infants. At the phylum level, probiotic treatment strongly increased the abundance of Firmicutes, whereas that of Proteobacteria was significantly reduced. At the family level, Streptococcaceae and Lactobacillaceae became prevalent after probiotic treatment, while the relative abundance of Enterobacteriaceae was reduced in the meantime. Most notably, significant correlations were observed between Lactobacillaceae abundance and serum cytokine levels. Further studies are required to shed more light on the characteristics of gut microbiota of VLBW neonates. And the modulation of microbiota should be considered to improve the survival rate of VLBW infants. |
| format | Article |
| id | doaj-art-bd451cedca7c4a34aa39d57b02d21dc0 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-bd451cedca7c4a34aa39d57b02d21dc02025-02-03T07:23:34ZengWileyGastroenterology Research and Practice1687-61211687-630X2019-01-01201910.1155/2019/36828363682836Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight InfantsYue-feng Li0Chuan-rui Zhu1Xue-lei Gong2Hui-ling Li3Li-kuan Xiong4Ke-jian Wang5Guo-Sheng Liu6Department of Pediatrics, The First Affiliated Hospital, Jinan University, Guangzhou 510630, ChinaDepartment of Pediatrics, The First Affiliated Hospital, Jinan University, Guangzhou 510630, ChinaDepartment of Pediatrics, Shenzhen Luohu Maternity and Child Health Hospital, 518019, ChinaDepartment of Neonatology, Bao’an Maternal and Child Health Hospital, Shenzhen 518133, ChinaCentral Laboratory, Bao’an Maternal and Child Health Hospital, Shenzhen 518133, ChinaLin He’s Academician Workstation of New Medicine and Clinical Translation, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, ChinaDepartment of Pediatrics, The First Affiliated Hospital, Jinan University, Guangzhou 510630, ChinaThe very low birth weight (VLBW) infant is at great risk for marked dysbiosis of the gut microbiota. In the present study, a total of 36 VLBW infants were randomly divided into two groups, who were treated with combined probiotics and placebo, and 72 fecal specimens on days 14 and 28 of life were collected from them. Finally, 32 fecal specimens extracted from 16 preterm VLBW infants were qualified and analyzed using 16S rRNA gene sequencing. The primary outcome was to evaluate the change of gut microbiota in VLBW infants after combined probiotic supplement. The secondary outcome was to analyze the correlation gut microbial composition and levels of cytokines. We found that probiotic treatment, but not placebo, decreased the α-diversity of gut microbiota in VLBW infants. At the phylum level, probiotic treatment strongly increased the abundance of Firmicutes, whereas that of Proteobacteria was significantly reduced. At the family level, Streptococcaceae and Lactobacillaceae became prevalent after probiotic treatment, while the relative abundance of Enterobacteriaceae was reduced in the meantime. Most notably, significant correlations were observed between Lactobacillaceae abundance and serum cytokine levels. Further studies are required to shed more light on the characteristics of gut microbiota of VLBW neonates. And the modulation of microbiota should be considered to improve the survival rate of VLBW infants.http://dx.doi.org/10.1155/2019/3682836 |
| spellingShingle | Yue-feng Li Chuan-rui Zhu Xue-lei Gong Hui-ling Li Li-kuan Xiong Ke-jian Wang Guo-Sheng Liu Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants Gastroenterology Research and Practice |
| title | Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants |
| title_full | Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants |
| title_fullStr | Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants |
| title_full_unstemmed | Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants |
| title_short | Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants |
| title_sort | beneficial effects of probiotic treatment on gut microbiota in very low birth weight infants |
| url | http://dx.doi.org/10.1155/2019/3682836 |
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