The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available at
The proteins encoded by Influenza A virus (IAV) evade the innate immune system through diverse strategies to facilitate their replication. However, the regulatory mechanisms remain not fully understood. In this study, we identified that the H9N2 PB1 protein suppressed the activities of the IFN-β, IS...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124012173 |
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| author | Jiawei Luo Yiyuan Lu Enqi Dai Nianchun Yin Ting Wang Hongxi Qian Qingrong Jiang Xin Cao Chunfeng Wang Yan Zeng |
| author_facet | Jiawei Luo Yiyuan Lu Enqi Dai Nianchun Yin Ting Wang Hongxi Qian Qingrong Jiang Xin Cao Chunfeng Wang Yan Zeng |
| author_sort | Jiawei Luo |
| collection | DOAJ |
| description | The proteins encoded by Influenza A virus (IAV) evade the innate immune system through diverse strategies to facilitate their replication. However, the regulatory mechanisms remain not fully understood. In this study, we identified that the H9N2 PB1 protein suppressed the activities of the IFN-β, ISRE, and NF-κB promoters. Furthermore, H9N2 PB1 inhibited the phosphorylation of IRF3, IκBα, and TBK1 and the secretion of IFN-β. The results demonstrated H9N2 PB1 as a negative regulator of the RIG-I signaling pathway. Subsequent investigations revealed a specific interaction between H9N2 PB1 and MAVS, which disturbed the stability of MAVS. Notably, we discovered that H9N2 PB1 exploited the function of TRIM25, leading to the autophagic degradation of MAVS through K48-linked polyubiquitination. In conclusion, we uncovered a negative regulatory axis consisting of H9N2 PB1-TRIM25-MAVS-IFN-I. These findings provide valuable insights into the molecular interactions involved in the regulation of the host's innate immune antiviral response by IAV. |
| format | Article |
| id | doaj-art-bd1f9665257f42a0a214094f349b8a1a |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-bd1f9665257f42a0a214094f349b8a1a2025-01-22T05:40:47ZengElsevierPoultry Science0032-57912025-01-011041104639The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available atJiawei Luo0Yiyuan Lu1Enqi Dai2Nianchun Yin3Ting Wang4Hongxi Qian5Qingrong Jiang6Xin Cao7Chunfeng Wang8Yan Zeng9College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, PR ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, 130118, PR China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, 130118, PR ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, PR ChinaSuining Municipal Agricultural and Rural Bureau of Sichuan Province, Suining, 629000, PR ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, PR ChinaTECHLEX Food Co. Ltd, Mianyang, 621000, PR ChinaSichuan Sundaily Farm Ecological Food Co. Ltd, Mianyang, 621000, PR ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, 130118, PR China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, 130118, PR China; Corresponding authors.College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, 130118, PR China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, 130118, PR China; Corresponding authors.College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, 130118, PR China; Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, 130118, PR China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, 130118, PR China; Corresponding authors.The proteins encoded by Influenza A virus (IAV) evade the innate immune system through diverse strategies to facilitate their replication. However, the regulatory mechanisms remain not fully understood. In this study, we identified that the H9N2 PB1 protein suppressed the activities of the IFN-β, ISRE, and NF-κB promoters. Furthermore, H9N2 PB1 inhibited the phosphorylation of IRF3, IκBα, and TBK1 and the secretion of IFN-β. The results demonstrated H9N2 PB1 as a negative regulator of the RIG-I signaling pathway. Subsequent investigations revealed a specific interaction between H9N2 PB1 and MAVS, which disturbed the stability of MAVS. Notably, we discovered that H9N2 PB1 exploited the function of TRIM25, leading to the autophagic degradation of MAVS through K48-linked polyubiquitination. In conclusion, we uncovered a negative regulatory axis consisting of H9N2 PB1-TRIM25-MAVS-IFN-I. These findings provide valuable insights into the molecular interactions involved in the regulation of the host's innate immune antiviral response by IAV.http://www.sciencedirect.com/science/article/pii/S0032579124012173H9N2 PB1MAVSTRIM25Autophagic degradationK48-linked polyubiquitination |
| spellingShingle | Jiawei Luo Yiyuan Lu Enqi Dai Nianchun Yin Ting Wang Hongxi Qian Qingrong Jiang Xin Cao Chunfeng Wang Yan Zeng The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available at Poultry Science H9N2 PB1 MAVS TRIM25 Autophagic degradation K48-linked polyubiquitination |
| title | The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available at |
| title_full | The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available at |
| title_fullStr | The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available at |
| title_full_unstemmed | The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available at |
| title_short | The PB1 protein of H9N2 influenza A virus inhibits antiviral innate immunity by targeting MAVS for TRIM25-mediated autophagic degradationThe parent data and images support the findings of this work freely available at |
| title_sort | pb1 protein of h9n2 influenza a virus inhibits antiviral innate immunity by targeting mavs for trim25 mediated autophagic degradationthe parent data and images support the findings of this work freely available at |
| topic | H9N2 PB1 MAVS TRIM25 Autophagic degradation K48-linked polyubiquitination |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124012173 |
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