Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus
One hundred and four clinical specimens from provincial public health laboratories were tested for antibody to hepatitis C virus (HCV) envelope protein (anti-E2). To evaluate the effect of hypervariability of E2 region on anti-E2 assay, 49 recombinant immunoblot assay (RIBA) 3.0 positive samples wer...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wiley
1997-01-01
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| Series: | Canadian Journal of Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/1997/386268 |
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| _version_ | 1832549467703738368 |
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| author | RK Chaudhary E Burres |
| author_facet | RK Chaudhary E Burres |
| author_sort | RK Chaudhary |
| collection | DOAJ |
| description | One hundred and four clinical specimens from provincial public health laboratories were tested for antibody to hepatitis C virus (HCV) envelope protein (anti-E2). To evaluate the effect of hypervariability of E2 region on anti-E2 assay, 49 recombinant immunoblot assay (RIBA) 3.0 positive samples were genotyped. All 49 genotyped samples were positive for anti-E2. Eight of 12 (67%) indeterminate, HCV RNA positive samples were anti-E2 reactive. Nine of 30 (30%) indeterminate, HCV RNA negative samples were also positive for anti-E2. Anti-E2 was detected in two of 13 (15%) RIBA-negative and enzyme immunoassays-positive samples. Although small number of samples were tested, the results showed that it may be possible to resolve indeterminate samples with the anti-E2 assay. |
| format | Article |
| id | doaj-art-bd138db54e1540c8bcdd7e2da71f7b0f |
| institution | Kabale University |
| issn | 1180-2332 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases |
| spelling | doaj-art-bd138db54e1540c8bcdd7e2da71f7b0f2025-02-03T06:11:20ZengWileyCanadian Journal of Infectious Diseases1180-23321997-01-018422923110.1155/1997/386268Detection of Antibody to Envelope (E2) Antigen of Hepatitis C VirusRK Chaudhary0E Burres1Laboratory for Viral Hepatitis, Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario, CanadaDiagnostic Division, Abbott Laboratories, North Chicago, Illinois, USAOne hundred and four clinical specimens from provincial public health laboratories were tested for antibody to hepatitis C virus (HCV) envelope protein (anti-E2). To evaluate the effect of hypervariability of E2 region on anti-E2 assay, 49 recombinant immunoblot assay (RIBA) 3.0 positive samples were genotyped. All 49 genotyped samples were positive for anti-E2. Eight of 12 (67%) indeterminate, HCV RNA positive samples were anti-E2 reactive. Nine of 30 (30%) indeterminate, HCV RNA negative samples were also positive for anti-E2. Anti-E2 was detected in two of 13 (15%) RIBA-negative and enzyme immunoassays-positive samples. Although small number of samples were tested, the results showed that it may be possible to resolve indeterminate samples with the anti-E2 assay.http://dx.doi.org/10.1155/1997/386268 |
| spellingShingle | RK Chaudhary E Burres Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus Canadian Journal of Infectious Diseases |
| title | Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus |
| title_full | Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus |
| title_fullStr | Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus |
| title_full_unstemmed | Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus |
| title_short | Detection of Antibody to Envelope (E2) Antigen of Hepatitis C Virus |
| title_sort | detection of antibody to envelope e2 antigen of hepatitis c virus |
| url | http://dx.doi.org/10.1155/1997/386268 |
| work_keys_str_mv | AT rkchaudhary detectionofantibodytoenvelopee2antigenofhepatitiscvirus AT eburres detectionofantibodytoenvelopee2antigenofhepatitiscvirus |