4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos

Summary: As light sheet fluorescence microscopy (LSFM) becomes widely available, reconstruction of time-lapse imaging will further our understanding of complex biological processes at cellular resolution. Here, we present a comprehensive workflow for in toto capture, processing, and analysis of mult...

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Bibliographic Details
Main Authors: Martin H. Dominguez, Jonathon M. Muncie-Vasic, Benoit G. Bruneau
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006804
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