Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells
The human leukocyte antigen G (HLA-G) is an immune checkpoint molecule with a complex network of interactions with several inhibitory receptors. Although the effect of HLA-G on T cells and NK cells is well studied, the effect of HLA-G on B cells is still largely elusive. B cells are of particular in...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/4829227 |
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| author | Hana Rohn Cordula Lang Sabine Schramm Falko M. Heinemann Mirko Trilling Anja Gäckler Oliver Witzke Peter A. Horn Vera Rebmann |
| author_facet | Hana Rohn Cordula Lang Sabine Schramm Falko M. Heinemann Mirko Trilling Anja Gäckler Oliver Witzke Peter A. Horn Vera Rebmann |
| author_sort | Hana Rohn |
| collection | DOAJ |
| description | The human leukocyte antigen G (HLA-G) is an immune checkpoint molecule with a complex network of interactions with several inhibitory receptors. Although the effect of HLA-G on T cells and NK cells is well studied, the effect of HLA-G on B cells is still largely elusive. B cells are of particular interest in the context of the HLA-G-ILT-2 interaction because the ILT-2 receptor is constitutively expressed on most B cells, whereas it is only present on some subsets of T and NK cells. To characterize the effect of HLA-G5 molecules on B cells, we studied splenic B cells derived from cytomegalovirus (CMV) sero-positive donors after CMV stimulation with antigens in the presence and absence of soluble HLA-G5. In the presence of HLA-G5, increased expression of the ITIM-bearing Ig-like transcript (ILT-2) was observed on B cells, but its expression was not affected by stimulation with CMV antigens. Moreover, it became evident that HLA-G5 exposure resulted in a decreased expression of CD27 and CD38 and, accordingly, in lower proportions of CD19+CD27+CD38+ and higher proportions of CD19+CD27-CD38- B cells. Taken together, our in vitro findings demonstrate that soluble HLA-G5 suppresses markers of B cell activation, suggesting that HLA-G5 has an impact on splenic B cell differentiation and activation. Based on these results, further investigation regarding the role of HLA-G as a prognostic factor and a potential therapeutic agent with respect to B cell function appears reasonable. |
| format | Article |
| id | doaj-art-bc40e9f5db4146bba090e582ad7e2ec0 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-bc40e9f5db4146bba090e582ad7e2ec02025-02-03T01:08:47ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/4829227Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cellsHana Rohn0Cordula Lang1Sabine Schramm2Falko M. Heinemann3Mirko Trilling4Anja Gäckler5Oliver Witzke6Peter A. Horn7Vera Rebmann8Department of Infectious Diseases. West German Centre of Infectious DiseasesInstitute for Transfusion MedicineInstitute for Transfusion MedicineInstitute for Transfusion MedicineInstitute for VirologyDepartment of NephrologyDepartment of Infectious Diseases. West German Centre of Infectious DiseasesInstitute for Transfusion MedicineInstitute for Transfusion MedicineThe human leukocyte antigen G (HLA-G) is an immune checkpoint molecule with a complex network of interactions with several inhibitory receptors. Although the effect of HLA-G on T cells and NK cells is well studied, the effect of HLA-G on B cells is still largely elusive. B cells are of particular interest in the context of the HLA-G-ILT-2 interaction because the ILT-2 receptor is constitutively expressed on most B cells, whereas it is only present on some subsets of T and NK cells. To characterize the effect of HLA-G5 molecules on B cells, we studied splenic B cells derived from cytomegalovirus (CMV) sero-positive donors after CMV stimulation with antigens in the presence and absence of soluble HLA-G5. In the presence of HLA-G5, increased expression of the ITIM-bearing Ig-like transcript (ILT-2) was observed on B cells, but its expression was not affected by stimulation with CMV antigens. Moreover, it became evident that HLA-G5 exposure resulted in a decreased expression of CD27 and CD38 and, accordingly, in lower proportions of CD19+CD27+CD38+ and higher proportions of CD19+CD27-CD38- B cells. Taken together, our in vitro findings demonstrate that soluble HLA-G5 suppresses markers of B cell activation, suggesting that HLA-G5 has an impact on splenic B cell differentiation and activation. Based on these results, further investigation regarding the role of HLA-G as a prognostic factor and a potential therapeutic agent with respect to B cell function appears reasonable.http://dx.doi.org/10.1155/2022/4829227 |
| spellingShingle | Hana Rohn Cordula Lang Sabine Schramm Falko M. Heinemann Mirko Trilling Anja Gäckler Oliver Witzke Peter A. Horn Vera Rebmann Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells Journal of Immunology Research |
| title | Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells |
| title_full | Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells |
| title_fullStr | Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells |
| title_full_unstemmed | Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells |
| title_short | Effect of HLA-G5 Immune Checkpoint Molecule on the Expression of ILT-2, CD27, and CD38 in Splenic B cells |
| title_sort | effect of hla g5 immune checkpoint molecule on the expression of ilt 2 cd27 and cd38 in splenic b cells |
| url | http://dx.doi.org/10.1155/2022/4829227 |
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