Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling
We report a principle prototype of space animal cell perfusion culture and observation. Unlike previous work, our cell culture system cannot only realize microfluidic and temperature controlling, automatic observation, and recording but also meet an increasing cell culture at large scale operation a...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Applied Mathematics |
| Online Access: | http://dx.doi.org/10.1155/2013/378253 |
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| author | Shangchun Fan Jinhao Sun Weiwei Xing Cheng Li Dongxue Wang |
| author_facet | Shangchun Fan Jinhao Sun Weiwei Xing Cheng Li Dongxue Wang |
| author_sort | Shangchun Fan |
| collection | DOAJ |
| description | We report a principle prototype of space animal cell perfusion culture and observation. Unlike previous work, our cell culture system cannot only realize microfluidic and temperature controlling, automatic observation, and recording but also meet an increasing cell culture at large scale operation and overcome shear force for animal cells. A key component in the system is ingenious structural fused silica cell culture cavity with the wedge-shaped connection. Finite volume method (FVM) is applied to calculate its multipoint flow field, pressure field, axial velocity, tangential velocity, and radial velocity. In order to provide appropriate flow rate, temperature, and shear force for space animal cell culture, a closed-loop microfluidic circuit and proportional, integrating, and differentiation (PID) algorithm are employed. This paper also illustrates system architecture and operating method of the principle prototype. The dynamic culture, autofocus observation, and recording of M763 cells are performed successfully within 72 h in the laboratory environment. This research can provide a reference for space flight mission that carries an apparatus with similar functions. |
| format | Article |
| id | doaj-art-bc39b2197c094936bcc3869a65ec9a69 |
| institution | Kabale University |
| issn | 1110-757X 1687-0042 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Applied Mathematics |
| spelling | doaj-art-bc39b2197c094936bcc3869a65ec9a692025-02-03T01:29:10ZengWileyJournal of Applied Mathematics1110-757X1687-00422013-01-01201310.1155/2013/378253378253Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature ControllingShangchun Fan0Jinhao Sun1Weiwei Xing2Cheng Li3Dongxue Wang4School of Instrument Science and Opto-Electronics Engineering, Beihang University, 37 Xueyuan Road, Haidian District, Beijing 100191, ChinaSchool of Instrument Science and Opto-Electronics Engineering, Beihang University, 37 Xueyuan Road, Haidian District, Beijing 100191, ChinaSchool of Instrument Science and Opto-Electronics Engineering, Beihang University, 37 Xueyuan Road, Haidian District, Beijing 100191, ChinaSchool of Instrument Science and Opto-Electronics Engineering, Beihang University, 37 Xueyuan Road, Haidian District, Beijing 100191, ChinaSchool of Instrument Science and Opto-Electronics Engineering, Beihang University, 37 Xueyuan Road, Haidian District, Beijing 100191, ChinaWe report a principle prototype of space animal cell perfusion culture and observation. Unlike previous work, our cell culture system cannot only realize microfluidic and temperature controlling, automatic observation, and recording but also meet an increasing cell culture at large scale operation and overcome shear force for animal cells. A key component in the system is ingenious structural fused silica cell culture cavity with the wedge-shaped connection. Finite volume method (FVM) is applied to calculate its multipoint flow field, pressure field, axial velocity, tangential velocity, and radial velocity. In order to provide appropriate flow rate, temperature, and shear force for space animal cell culture, a closed-loop microfluidic circuit and proportional, integrating, and differentiation (PID) algorithm are employed. This paper also illustrates system architecture and operating method of the principle prototype. The dynamic culture, autofocus observation, and recording of M763 cells are performed successfully within 72 h in the laboratory environment. This research can provide a reference for space flight mission that carries an apparatus with similar functions.http://dx.doi.org/10.1155/2013/378253 |
| spellingShingle | Shangchun Fan Jinhao Sun Weiwei Xing Cheng Li Dongxue Wang Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling Journal of Applied Mathematics |
| title | Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling |
| title_full | Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling |
| title_fullStr | Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling |
| title_full_unstemmed | Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling |
| title_short | Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling |
| title_sort | design and simulation of a fused silica space cell culture and observation cavity with microfluidic and temperature controlling |
| url | http://dx.doi.org/10.1155/2013/378253 |
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