Histological characteristics and markers of proliferation and differentiation in rat brain with experimental glioma
Background/Aim. The cell line C6 is a continuous cell line of rat glioma and, as a transplantable line, is frequently used for induction into in vivo model of primary brain tumor. It is believed that, pursuant to its histological traits and biological behavior, this experimental tumor corre...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2014-01-01
|
| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2014/0042-84501400044R.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background/Aim. The cell line C6 is a continuous cell line of rat glioma and,
as a transplantable line, is frequently used for induction into in vivo model
of primary brain tumor. It is believed that, pursuant to its histological
traits and biological behavior, this experimental tumor corresponds to human
anaplastic astrocytoma of grade II/III, which is characterized by
proliferative and invasive potency, and marked cell differentiation. The aim
of this study was to determine macroscopic analysis of rat brain with
implanted tumor during tumorigenesis, histological features of tumor cells of
induced brain tumor and markers of proliferation (proliferation cell nuclear
antigen - PCNA, cytokeratin - CK 19) and differentiation (glial fibrillary
acidic protein -GFAP) in rat brain with implanted tumor. Methods. To
determine histological structure of the brain with implanted C6 cells, we
used brain sections stained for hematoxylin-eosin or kresyl violet, whereas
other sections were immunohistochemically stained for GFAP, CK 19 and PCNA.
Results. A statistically significant difference in weights of the left and
right brain hemispheres with implanted tumors during tumorigenesis in as soon
as 7 days from the day of inducing tumors was revealed. The tumor was of
cellular type, with distinct pleomorphism of cells and frequent
hyperchromasia of the nucleus. Immunohistochemical staining for PCNA revealed
a significant number of positive cells on the days 7, 14 and 21 day following
the implantation of C6 cells. CK 19 positive cells were present in both brain
hemispheres, and numerous GFAP positive astrocytes were found around the
puncture lesion. Conclusions. Within the experimental conditions of the
present research, C6 glioma did not demonstrate any relevant deviations
concerning development, clinical symptomatology and macroscopic anatomy
relative to those already described in the literature. |
|---|---|
| ISSN: | 0042-8450 |