Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions
A reverse phase HPLC method is described for the determination of Dorzolamide in eye drops. Chromatography was carried on an Inertsil ODS 3V column using Acetonitrile : (0.02M) 1,Octane Sulphonic acid buffer (pH 3.5) (36:64 v/v) on isocratic mode at a flow rate of 1 mL/min with UV detection at 254 n...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/258261 |
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| author | A. Narendra D. Deepika M. Mathrusri Annapurna |
| author_facet | A. Narendra D. Deepika M. Mathrusri Annapurna |
| author_sort | A. Narendra |
| collection | DOAJ |
| description | A reverse phase HPLC method is described for the determination of Dorzolamide in eye drops. Chromatography was carried on an Inertsil ODS 3V column using Acetonitrile : (0.02M) 1,Octane Sulphonic acid buffer (pH 3.5) (36:64 v/v) on isocratic mode at a flow rate of 1 mL/min with UV detection at 254 nm. The detector response was linear in the concentration range 4-720 µg/mL. The limit of detection and limit of quantification are found to be 0.7041 and 2.3483 µg/mL respectively. The method was validated as per the ICH guidelines. The proposed method is rapid, accurate and precise and can be applied for the routine analysis of dorzolamide in ophthalimic solutions. |
| format | Article |
| id | doaj-art-bc1c553137f64eb4b0f6f83d252069cb |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-bc1c553137f64eb4b0f6f83d252069cb2025-02-03T01:31:29ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-01931238124310.1155/2012/258261Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic SolutionsA. Narendra0D. Deepika1M. Mathrusri Annapurna2Department of Pharmaceutical Analysis and Quality Assurance, Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, IndiaRoyal College of Pharmacy and Health Sciences, Berhampur, Orissa, IndiaGITAM Institute of Pharmacy, GITAM University, Visakhapatnam (A.P.), IndiaA reverse phase HPLC method is described for the determination of Dorzolamide in eye drops. Chromatography was carried on an Inertsil ODS 3V column using Acetonitrile : (0.02M) 1,Octane Sulphonic acid buffer (pH 3.5) (36:64 v/v) on isocratic mode at a flow rate of 1 mL/min with UV detection at 254 nm. The detector response was linear in the concentration range 4-720 µg/mL. The limit of detection and limit of quantification are found to be 0.7041 and 2.3483 µg/mL respectively. The method was validated as per the ICH guidelines. The proposed method is rapid, accurate and precise and can be applied for the routine analysis of dorzolamide in ophthalimic solutions.http://dx.doi.org/10.1155/2012/258261 |
| spellingShingle | A. Narendra D. Deepika M. Mathrusri Annapurna Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions E-Journal of Chemistry |
| title | Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions |
| title_full | Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions |
| title_fullStr | Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions |
| title_full_unstemmed | Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions |
| title_short | Validated LC Method for the Estimation of Dorzolamide HCl (Carbonic Anhydrase Inhibitor) in Ophthalmic Solutions |
| title_sort | validated lc method for the estimation of dorzolamide hcl carbonic anhydrase inhibitor in ophthalmic solutions |
| url | http://dx.doi.org/10.1155/2012/258261 |
| work_keys_str_mv | AT anarendra validatedlcmethodfortheestimationofdorzolamidehclcarbonicanhydraseinhibitorinophthalmicsolutions AT ddeepika validatedlcmethodfortheestimationofdorzolamidehclcarbonicanhydraseinhibitorinophthalmicsolutions AT mmathrusriannapurna validatedlcmethodfortheestimationofdorzolamidehclcarbonicanhydraseinhibitorinophthalmicsolutions |