Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic Nephropathy
<i>Ishige okamurae</i> (<i>I. okamuare</i>), an edible brown alga, is rich in isophloroglucin A (IPA) phlorotannin compounds and is effective in preventing diseases, including diabetes. We evaluated its anti-glycation ability, intracellular reactive oxygen species scavenging...
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2025-01-01
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| author | Chi-Heung Cho Min-Gyeong Kim Bomi Ryu Sang-Hoon Lee |
| author_facet | Chi-Heung Cho Min-Gyeong Kim Bomi Ryu Sang-Hoon Lee |
| author_sort | Chi-Heung Cho |
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| description | <i>Ishige okamurae</i> (<i>I. okamuare</i>), an edible brown alga, is rich in isophloroglucin A (IPA) phlorotannin compounds and is effective in preventing diseases, including diabetes. We evaluated its anti-glycation ability, intracellular reactive oxygen species scavenging activity, inhibitory effect on the accumulation of intracellular MGO/MGO-derived advanced glycation end products (AGE), and regulation of downstream signaling pathways related to the AGE–receptor for AGEs (RAGE) interaction. IPA (0.2, 1, and 5 μM) demonstrated anti-glycation ability by inhibiting the formation of glucose-fructose-BSA-derived AGEs by up to 54.63% compared to the untreated control, reducing the formation of irreversible cross-links between MGO-derived AGEs and collagen by 67.68% and the breaking down of existing cross-links by approximately 91% (<i>p</i> < 0.001). IPA protected cells from MGO-induced oxidative stress by inhibiting intracellular MGO accumulation (untreated cells: 1.62 μg/mL, MGO treated cells: 25.27 μg/mL, and IPA 5 μM: 11.23 μg/mL) (<i>p</i> < 0.001) and AGE generation and inhibited MGO-induced renal cell damage via the downregulation of MGO-induced RAGE protein expression (relative protein expression levels of MGO treated cells: 9.37 and IPA 5 μM:1.74) (<i>p</i> < 0.001). Overall, these results suggest that IPA has the potential to be utilized as a useful natural agent for the prevention and management of AGE-related diabetic nephropathy, owing to its strong anti-glycation activity. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Marine Drugs |
| spelling | doaj-art-bbaf54054a204a0086bee8d621ea0b582025-01-24T13:39:37ZengMDPI AGMarine Drugs1660-33972025-01-012314810.3390/md23010048Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic NephropathyChi-Heung Cho0Min-Gyeong Kim1Bomi Ryu2Sang-Hoon Lee3Division of Functional Food Research, Korea Food Research Institute, Wanju-gun 55365, Republic of KoreaDivision of Functional Food Research, Korea Food Research Institute, Wanju-gun 55365, Republic of KoreaMajor of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of KoreaDivision of Functional Food Research, Korea Food Research Institute, Wanju-gun 55365, Republic of Korea<i>Ishige okamurae</i> (<i>I. okamuare</i>), an edible brown alga, is rich in isophloroglucin A (IPA) phlorotannin compounds and is effective in preventing diseases, including diabetes. We evaluated its anti-glycation ability, intracellular reactive oxygen species scavenging activity, inhibitory effect on the accumulation of intracellular MGO/MGO-derived advanced glycation end products (AGE), and regulation of downstream signaling pathways related to the AGE–receptor for AGEs (RAGE) interaction. IPA (0.2, 1, and 5 μM) demonstrated anti-glycation ability by inhibiting the formation of glucose-fructose-BSA-derived AGEs by up to 54.63% compared to the untreated control, reducing the formation of irreversible cross-links between MGO-derived AGEs and collagen by 67.68% and the breaking down of existing cross-links by approximately 91% (<i>p</i> < 0.001). IPA protected cells from MGO-induced oxidative stress by inhibiting intracellular MGO accumulation (untreated cells: 1.62 μg/mL, MGO treated cells: 25.27 μg/mL, and IPA 5 μM: 11.23 μg/mL) (<i>p</i> < 0.001) and AGE generation and inhibited MGO-induced renal cell damage via the downregulation of MGO-induced RAGE protein expression (relative protein expression levels of MGO treated cells: 9.37 and IPA 5 μM:1.74) (<i>p</i> < 0.001). Overall, these results suggest that IPA has the potential to be utilized as a useful natural agent for the prevention and management of AGE-related diabetic nephropathy, owing to its strong anti-glycation activity.https://www.mdpi.com/1660-3397/23/1/48brown seaweeddiabetic complicationphlorotanninmethylglyoxal-derived advanced glycation end productsmouse glomerular mesangial cells |
| spellingShingle | Chi-Heung Cho Min-Gyeong Kim Bomi Ryu Sang-Hoon Lee Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic Nephropathy Marine Drugs brown seaweed diabetic complication phlorotannin methylglyoxal-derived advanced glycation end products mouse glomerular mesangial cells |
| title | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic Nephropathy |
| title_full | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic Nephropathy |
| title_fullStr | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic Nephropathy |
| title_full_unstemmed | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic Nephropathy |
| title_short | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Protects Glomerular Cells from Methylglyoxal-Induced Diacarbonyl Stress and Inhibits the Pathogenesis of Diabetic Nephropathy |
| title_sort | ishophloroglucin a isolated from i ishige okamurae i protects glomerular cells from methylglyoxal induced diacarbonyl stress and inhibits the pathogenesis of diabetic nephropathy |
| topic | brown seaweed diabetic complication phlorotannin methylglyoxal-derived advanced glycation end products mouse glomerular mesangial cells |
| url | https://www.mdpi.com/1660-3397/23/1/48 |
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