A simple and reliable method for determination of optimum pH in coupled enzyme assays
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kina...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-04-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2019-0126 |
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| _version_ | 1850151814090981376 |
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| author | Lee Bowman Rachael Motamed Paul Lee Kadijah Aleem Astha S Berawala Katherine L Hayden David J Bzik Debasish Chattopadhyay |
| author_facet | Lee Bowman Rachael Motamed Paul Lee Kadijah Aleem Astha S Berawala Katherine L Hayden David J Bzik Debasish Chattopadhyay |
| author_sort | Lee Bowman |
| collection | DOAJ |
| description | Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kinase/lactate dehydrogenase coupled enzyme assay. This simple and reliable method can be broadly adapted to determine the pH optimum for various enzymes that are assayed using a coupled enzyme assay. |
| format | Article |
| id | doaj-art-bb7edf781d0e48d7a88e00b88cf5651f |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-bb7edf781d0e48d7a88e00b88cf5651f2025-08-20T02:26:08ZengTaylor & Francis GroupBioTechniques0736-62051940-98182020-04-0168420020310.2144/btn-2019-0126A simple and reliable method for determination of optimum pH in coupled enzyme assaysLee Bowman0Rachael Motamed1Paul Lee2Kadijah Aleem3Astha S Berawala4Katherine L Hayden5David J Bzik6Debasish Chattopadhyay71Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA1Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA2Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA2Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA1Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA1Department of Chemistry & Physics, Birmingham-Southern College, Birmingham, AL 35254, USA3Department of Microbiology & Immunology, Dartmouth Geisel School of Medicine, Lebanon, NH 03756, USA2Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USADetermination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kinase/lactate dehydrogenase coupled enzyme assay. This simple and reliable method can be broadly adapted to determine the pH optimum for various enzymes that are assayed using a coupled enzyme assay.https://www.future-science.com/doi/10.2144/btn-2019-0126coupled enzyme assayCryptosporidium parvumenzyme kineticslactate dehydrogenasepH optimizationPlasmodium falciparum |
| spellingShingle | Lee Bowman Rachael Motamed Paul Lee Kadijah Aleem Astha S Berawala Katherine L Hayden David J Bzik Debasish Chattopadhyay A simple and reliable method for determination of optimum pH in coupled enzyme assays BioTechniques coupled enzyme assay Cryptosporidium parvum enzyme kinetics lactate dehydrogenase pH optimization Plasmodium falciparum |
| title | A simple and reliable method for determination of optimum pH in coupled enzyme assays |
| title_full | A simple and reliable method for determination of optimum pH in coupled enzyme assays |
| title_fullStr | A simple and reliable method for determination of optimum pH in coupled enzyme assays |
| title_full_unstemmed | A simple and reliable method for determination of optimum pH in coupled enzyme assays |
| title_short | A simple and reliable method for determination of optimum pH in coupled enzyme assays |
| title_sort | simple and reliable method for determination of optimum ph in coupled enzyme assays |
| topic | coupled enzyme assay Cryptosporidium parvum enzyme kinetics lactate dehydrogenase pH optimization Plasmodium falciparum |
| url | https://www.future-science.com/doi/10.2144/btn-2019-0126 |
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