A simple and reliable method for determination of optimum pH in coupled enzyme assays

A simple and reliable method for determination of optimum pH in coupled enzyme assays

Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kina...

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Bibliographic Details
Main Authors: Lee Bowman, Rachael Motamed, Paul Lee, Kadijah Aleem, Astha S Berawala, Katherine L Hayden, David J Bzik, Debasish Chattopadhyay
Format: Article
Language:English
Published: Taylor & Francis Group 2020-04-01
Series:BioTechniques
Subjects:
coupled enzyme assay
Cryptosporidium parvum
enzyme kinetics
lactate dehydrogenase
pH optimization
Plasmodium falciparum
Online Access:https://www.future-science.com/doi/10.2144/btn-2019-0126
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https://www.future-science.com/doi/10.2144/btn-2019-0126

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