Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC)
Cymbopogon citratus (lemon grass) is commonly used in traditional folk medicine. The essential oil extracted from C. citratus has been reported as a potential anti-oxidant and anti-inflammatory agent. This study has been designed to explore the protective effect of C. citratus (lemon grass) against...
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Tsinghua University Press
2017-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453016300933 |
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| author | Jamuna S. Sakeena Sadullah M.S. Ashokkumar R. Gokul Shanmuganathan Senguttuvan Sivan Mozhi Niranjali Devaraj S. |
| author_facet | Jamuna S. Sakeena Sadullah M.S. Ashokkumar R. Gokul Shanmuganathan Senguttuvan Sivan Mozhi Niranjali Devaraj S. |
| author_sort | Jamuna S. |
| collection | DOAJ |
| description | Cymbopogon citratus (lemon grass) is commonly used in traditional folk medicine. The essential oil extracted from C. citratus has been reported as a potential anti-oxidant and anti-inflammatory agent. This study has been designed to explore the protective effect of C. citratus (lemon grass) against modified LDL (OxLDL and H2O2 LDL) induced cytotoxicity in Peripheral Blood Mononuclear Cells (PBMC). The essential oil extracted from C. citratus (EOC) was subjected to FT-IR spectroscopic analysis for the identification of functional groups. In vitro antioxidant assays were carried out to assess the electron donating capability of EOC as compared with a known standard L-ascorbic acid. The cytoprotective effects of EOC were determined in PBMC induced with modified LDL. Spectra obtained from FT-IR analysis showed the presence of functional groups in EOC such as H-bonded, OH stretching, NH stretching, aldehydeCH stretching, aldehyde/ketoneCO stretching, CC-stretching, CH3 bending, CH in plane bending. EOC has greater antioxidant property when compared with the standard L-ascorbic acid. EOC at all test concentrations demonstrated free radical scavenging activity and cytoprotective effect when challenged against modified LDL in PBMC. The above results show EOC as a promising antioxidant and cytoprotective agent. |
| format | Article |
| id | doaj-art-bb2d18a69d9f48528c1f0847dbebf561 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-bb2d18a69d9f48528c1f0847dbebf5612025-02-02T23:24:41ZengTsinghua University PressFood Science and Human Wellness2213-45302017-06-0162606910.1016/j.fshw.2017.02.001Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC)Jamuna S.0Sakeena Sadullah M.S.1Ashokkumar R.2Gokul Shanmuganathan3Senguttuvan Sivan Mozhi4Niranjali Devaraj S.5Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, IndiaDepartment of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, IndiaDepartment of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, IndiaDepartment of Biotechnology, Prist University, Puducherry, IndiaDepartment of Biotechnology, Prist University, Puducherry, IndiaDepartment of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, IndiaCymbopogon citratus (lemon grass) is commonly used in traditional folk medicine. The essential oil extracted from C. citratus has been reported as a potential anti-oxidant and anti-inflammatory agent. This study has been designed to explore the protective effect of C. citratus (lemon grass) against modified LDL (OxLDL and H2O2 LDL) induced cytotoxicity in Peripheral Blood Mononuclear Cells (PBMC). The essential oil extracted from C. citratus (EOC) was subjected to FT-IR spectroscopic analysis for the identification of functional groups. In vitro antioxidant assays were carried out to assess the electron donating capability of EOC as compared with a known standard L-ascorbic acid. The cytoprotective effects of EOC were determined in PBMC induced with modified LDL. Spectra obtained from FT-IR analysis showed the presence of functional groups in EOC such as H-bonded, OH stretching, NH stretching, aldehydeCH stretching, aldehyde/ketoneCO stretching, CC-stretching, CH3 bending, CH in plane bending. EOC has greater antioxidant property when compared with the standard L-ascorbic acid. EOC at all test concentrations demonstrated free radical scavenging activity and cytoprotective effect when challenged against modified LDL in PBMC. The above results show EOC as a promising antioxidant and cytoprotective agent.http://www.sciencedirect.com/science/article/pii/S2213453016300933Cymbopogon citratusAntioxidantsCytoprotectivePBMCModified LDL |
| spellingShingle | Jamuna S. Sakeena Sadullah M.S. Ashokkumar R. Gokul Shanmuganathan Senguttuvan Sivan Mozhi Niranjali Devaraj S. Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC) Food Science and Human Wellness Cymbopogon citratus Antioxidants Cytoprotective PBMC Modified LDL |
| title | Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC) |
| title_full | Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC) |
| title_fullStr | Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC) |
| title_full_unstemmed | Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC) |
| title_short | Potential antioxidant and cytoprotective effects of essential oil extracted from Cymbopogon citratus on OxLDL and H2O2 LDL induced Human Peripheral Blood Mononuclear Cells (PBMC) |
| title_sort | potential antioxidant and cytoprotective effects of essential oil extracted from cymbopogon citratus on oxldl and h2o2 ldl induced human peripheral blood mononuclear cells pbmc |
| topic | Cymbopogon citratus Antioxidants Cytoprotective PBMC Modified LDL |
| url | http://www.sciencedirect.com/science/article/pii/S2213453016300933 |
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