Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma
Abstract Glioblastoma multiforme (GBM) is the most prevalent, treatment-resistant, and fatal form of brain malignancy. It is characterized by genetic heterogeneity, and an infiltrative nature, and GBM treatment is highly challenging. Despite multimodal therapies, clinicians lack efficient prognostic...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83800-x |
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| author | Elham Ali Marwa Adel Ahmed May A. Shawki Lobna R. Ezz El Arab Mohamed K. Khalifa Menha Swellam |
| author_facet | Elham Ali Marwa Adel Ahmed May A. Shawki Lobna R. Ezz El Arab Mohamed K. Khalifa Menha Swellam |
| author_sort | Elham Ali |
| collection | DOAJ |
| description | Abstract Glioblastoma multiforme (GBM) is the most prevalent, treatment-resistant, and fatal form of brain malignancy. It is characterized by genetic heterogeneity, and an infiltrative nature, and GBM treatment is highly challenging. Despite multimodal therapies, clinicians lack efficient prognostic and predictive markers. Therefore, new insights into GBM management are urgently needed to increase the chance of therapeutic success. Circulating miRNAs (miRs) are important regulators of cancer progression and are potentially useful for GBM diagnosis and treatment. This study investigated how miR-29a, miR-106a, and miR-200a affect the prognosis of GBM patients. This study was conducted on 25 GBM patients and 20 healthy volunteers as a control group. The expression levels of target miRs were analyzed pre- and post-treatment using qRT-PCR and evaluated in relation to both clinical GBM criteria and the patient’s survival modes. The diagnostic efficacy of target miRs was assessed using the receiver operating characteristic (ROC) curve. MiRs levels showed significant differences among the enrolled participants. All investigated miRs were significantly elevated in GBM patients with non-frontal lesions. Only miR-200a showed a significant difference in GBM patients older than 60 years with a tumor size ≥ 5 mm. Regarding miR-106a, a significant difference was detected based on the surgical strategy and use of an Eastern Cooperative Oncology Group (ECOG) performance status equal to 2. For miR-29a, a significant upregulation was detected according to the surgical strategy. All post-treatment miRs levels in GBM patients were significantly downregulated. In conclusion, circulating miRs revealed a significant role in predicting GBM patient treatment outcomes providing valuable insights for personalized therapeutic strategies. |
| format | Article |
| id | doaj-art-b91bae3f20484d2b83aaafaa8f2520a2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b91bae3f20484d2b83aaafaa8f2520a22025-01-19T12:21:00ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-024-83800-xExpression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastomaElham Ali0Marwa Adel Ahmed1May A. Shawki2Lobna R. Ezz El Arab3Mohamed K. Khalifa4Menha Swellam5Molecular Biology, Zoology and Entomology Department, Faculty of Science (for Girls), Al-Azhar UniversityClinical Pharmacy Department, Faculty of Pharmacy, Ain Shams UniversityClinical Pharmacy Department, Faculty of Pharmacy, Ain Shams UniversityClinical Oncology Department, Faculty of Medicine, Ain Shams UniversityCSO at OmicsenseBiochemistry Department, Biotechnology Research Institute, National Research CentreAbstract Glioblastoma multiforme (GBM) is the most prevalent, treatment-resistant, and fatal form of brain malignancy. It is characterized by genetic heterogeneity, and an infiltrative nature, and GBM treatment is highly challenging. Despite multimodal therapies, clinicians lack efficient prognostic and predictive markers. Therefore, new insights into GBM management are urgently needed to increase the chance of therapeutic success. Circulating miRNAs (miRs) are important regulators of cancer progression and are potentially useful for GBM diagnosis and treatment. This study investigated how miR-29a, miR-106a, and miR-200a affect the prognosis of GBM patients. This study was conducted on 25 GBM patients and 20 healthy volunteers as a control group. The expression levels of target miRs were analyzed pre- and post-treatment using qRT-PCR and evaluated in relation to both clinical GBM criteria and the patient’s survival modes. The diagnostic efficacy of target miRs was assessed using the receiver operating characteristic (ROC) curve. MiRs levels showed significant differences among the enrolled participants. All investigated miRs were significantly elevated in GBM patients with non-frontal lesions. Only miR-200a showed a significant difference in GBM patients older than 60 years with a tumor size ≥ 5 mm. Regarding miR-106a, a significant difference was detected based on the surgical strategy and use of an Eastern Cooperative Oncology Group (ECOG) performance status equal to 2. For miR-29a, a significant upregulation was detected according to the surgical strategy. All post-treatment miRs levels in GBM patients were significantly downregulated. In conclusion, circulating miRs revealed a significant role in predicting GBM patient treatment outcomes providing valuable insights for personalized therapeutic strategies.https://doi.org/10.1038/s41598-024-83800-xGlioblastomamiR-29amiR-106amiR-200aBiomarkersIn silico analysis |
| spellingShingle | Elham Ali Marwa Adel Ahmed May A. Shawki Lobna R. Ezz El Arab Mohamed K. Khalifa Menha Swellam Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma Scientific Reports Glioblastoma miR-29a miR-106a miR-200a Biomarkers In silico analysis |
| title | Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma |
| title_full | Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma |
| title_fullStr | Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma |
| title_full_unstemmed | Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma |
| title_short | Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma |
| title_sort | expression of some circulating micrornas as predictive biomarkers for prognosis and treatment response in glioblastoma |
| topic | Glioblastoma miR-29a miR-106a miR-200a Biomarkers In silico analysis |
| url | https://doi.org/10.1038/s41598-024-83800-x |
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