The siRNA Non-seed Region and Its Target Sequences Are Auxiliary Determinants of Off-Target Effects.

RNA interference (RNAi) is a powerful tool for post-transcriptional gene silencing. However, the siRNA guide strand may bind unintended off-target transcripts via partial sequence complementarity by a mechanism closely mirroring micro RNA (miRNA) silencing. To better understand these off-target effe...

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Bibliographic Details
Main Authors: Piotr J Kamola, Yuko Nakano, Tomoko Takahashi, Paul A Wilson, Kumiko Ui-Tei
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-12-01
Series:PLoS Computational Biology
Online Access:https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1004656&type=printable
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