An integrated protocol based on workflows of imaged capillary isoelectric focusing (icIEF) for in-depth protein heterogenous characterization: High-efficient fractionation and online mass spectrometry detection

An integrated protocol based on workflows of imaged capillary isoelectric focusing (icIEF) for in-depth protein heterogenous characterization: High-efficient fractionation and online mass spectrometry detection

We present an integrated protocol for studying the charge heterogeneity of therapeutic proteins combining two workflows: imaged capillary isoelectric focusing (icIEF) with fractionation and online mass spectrometry (MS) detection on a single platform. This protocol enables both intact MS-based prote...

Full description

Saved in:
Bibliographic Details
Main Authors: Teresa Kwok, She Lin Chan, Mike Zhou, Tong Chen, Niusheng Xu, Victor Li, Tiemin Huang, Tao Bo
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Green Analytical Chemistry
Subjects:
Imaged capillary isoelectric focusing
Integrated protocol
Protein heterogeneity
Fractionation
Mass spectrometry online
Online Access:http://www.sciencedirect.com/science/article/pii/S2772577425000011
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

http://www.sciencedirect.com/science/article/pii/S2772577425000011

Similar Items