Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines
In this study, the bioactivity-guided fractionation was conducted on the aerial parts of Arctium lappa L. and then the extracts were tested in vitro on breast cancer (MCF-7), colorectal cancer (HCT-116), and normal cells (EA.hy926). The n-hexane fraction (EHX) of the ethanolic extract showed strong...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2020/7286053 |
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| author | Mohamad Taleb Agha Hussein M. Baharetha Majed Ahmed Al-Mansoub Yasser M. Tabana Nur Hidayah Kaz Abdul Aziz Mun Fei Yam Amin Malik Shah Abdul Majid |
| author_facet | Mohamad Taleb Agha Hussein M. Baharetha Majed Ahmed Al-Mansoub Yasser M. Tabana Nur Hidayah Kaz Abdul Aziz Mun Fei Yam Amin Malik Shah Abdul Majid |
| author_sort | Mohamad Taleb Agha |
| collection | DOAJ |
| description | In this study, the bioactivity-guided fractionation was conducted on the aerial parts of Arctium lappa L. and then the extracts were tested in vitro on breast cancer (MCF-7), colorectal cancer (HCT-116), and normal cells (EA.hy926). The n-hexane fraction (EHX) of the ethanolic extract showed strong activity against both MCF-7 and EA.hy926 cell lines (IC50 values: 14.08 ± 3.64 and 27.25 ± 3.45 μg/mL, respectively). The proapoptotic activity of EHX was assessed using MCF-7. Morphological alterations were visualized using Hoechst staining and a transmission electron microscope. Cancer cell signal transduction pathways were investigated, and EHX significantly upregulated p53, TGF-β, and NF-κB. Furthermore, EHX was found to disrupt the metastatic cascade of breast cancer cells by the inhibition of cell proliferation, migration, invasion, and colonization. The antiangiogenic activity of EHX fraction showed potent inhibition of rat aorta microvessels with IC50 value: 4.34 ± 1.64 μg/mL. This result was supported by the downregulation of VEGF-A expression up to 54%. Over 20 compounds were identified in EHX using GC-MS, of which stigmasterol, ß-sitosterol, and 3-O-acetyllupeol are the major active compounds. Phytochemical analysis of EHX showed higher phenolic and flavonoid contents with a substantial antioxidant activity. In conclusion, this work demonstrated that A. lappa has valuable anticancer activity and antiangiogenic properties that might be useful in breast cancer therapy. |
| format | Article |
| id | doaj-art-b858325faba7432ca6de3d0582d9ee35 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-b858325faba7432ca6de3d0582d9ee352025-02-03T06:46:08ZengWileyScientifica2090-908X2020-01-01202010.1155/2020/72860537286053Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell LinesMohamad Taleb Agha0Hussein M. Baharetha1Majed Ahmed Al-Mansoub2Yasser M. Tabana3Nur Hidayah Kaz Abdul Aziz4Mun Fei Yam5Amin Malik Shah Abdul Majid6EMAN Testing & Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pinang, MalaysiaEMAN Testing & Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pinang, MalaysiaEMAN Testing & Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pinang, MalaysiaEMAN Testing & Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pinang, MalaysiaEMAN Testing & Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pinang, MalaysiaEMAN Testing & Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pinang, MalaysiaEMAN Testing & Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pinang, MalaysiaIn this study, the bioactivity-guided fractionation was conducted on the aerial parts of Arctium lappa L. and then the extracts were tested in vitro on breast cancer (MCF-7), colorectal cancer (HCT-116), and normal cells (EA.hy926). The n-hexane fraction (EHX) of the ethanolic extract showed strong activity against both MCF-7 and EA.hy926 cell lines (IC50 values: 14.08 ± 3.64 and 27.25 ± 3.45 μg/mL, respectively). The proapoptotic activity of EHX was assessed using MCF-7. Morphological alterations were visualized using Hoechst staining and a transmission electron microscope. Cancer cell signal transduction pathways were investigated, and EHX significantly upregulated p53, TGF-β, and NF-κB. Furthermore, EHX was found to disrupt the metastatic cascade of breast cancer cells by the inhibition of cell proliferation, migration, invasion, and colonization. The antiangiogenic activity of EHX fraction showed potent inhibition of rat aorta microvessels with IC50 value: 4.34 ± 1.64 μg/mL. This result was supported by the downregulation of VEGF-A expression up to 54%. Over 20 compounds were identified in EHX using GC-MS, of which stigmasterol, ß-sitosterol, and 3-O-acetyllupeol are the major active compounds. Phytochemical analysis of EHX showed higher phenolic and flavonoid contents with a substantial antioxidant activity. In conclusion, this work demonstrated that A. lappa has valuable anticancer activity and antiangiogenic properties that might be useful in breast cancer therapy.http://dx.doi.org/10.1155/2020/7286053 |
| spellingShingle | Mohamad Taleb Agha Hussein M. Baharetha Majed Ahmed Al-Mansoub Yasser M. Tabana Nur Hidayah Kaz Abdul Aziz Mun Fei Yam Amin Malik Shah Abdul Majid Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines Scientifica |
| title | Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines |
| title_full | Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines |
| title_fullStr | Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines |
| title_full_unstemmed | Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines |
| title_short | Proapoptotic and Antiangiogenic Activities of Arctium Lappa L. on Breast Cancer Cell Lines |
| title_sort | proapoptotic and antiangiogenic activities of arctium lappa l on breast cancer cell lines |
| url | http://dx.doi.org/10.1155/2020/7286053 |
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