Induced sputum is a reproducible method to assess airway inflammation in asthma
To evaluate the reproducibility of induced sputum analysis, and to estimate the sample size required to obtained reliable results, sputum was induced by hypertonic saline inhalation in 29 asthmatic subjects on two different days. The whole sample method was used for analysis, and inflammatory cells...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350210000015692 |
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| _version_ | 1832551072700301312 |
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| author | Elena Bacci Silvana Cianchetti Stefano Carnevali Maria Laura Bartoli Federico L. Dente Antonella Di Franco Daniele Giannini Barbara Vagaggini Pier Luigi Paggiaro |
| author_facet | Elena Bacci Silvana Cianchetti Stefano Carnevali Maria Laura Bartoli Federico L. Dente Antonella Di Franco Daniele Giannini Barbara Vagaggini Pier Luigi Paggiaro |
| author_sort | Elena Bacci |
| collection | DOAJ |
| description | To evaluate the reproducibility of induced sputum analysis, and to estimate the sample size required to obtained reliable results, sputum was induced by hypertonic saline inhalation in 29 asthmatic subjects on two different days. The whole sample method was used for analysis, and inflammatory cells were counted on cytospin slides. Reproducibility, expressed by intra-class correlation coefficients, was good for macrophages (+0.80), neutrophils (+0.85), and eosinophils (+0.87), but not for lymphocytes (+0.15). Detectable differences were 5.5% for macrophages, 0.6% for lymphocytes, 5.2% for neutrophils, and 3.0% for eosinophils. We conclude that analysis of induced sputum is a reproducible method to study airway inflammation in asthma. Sample sizes greater than ours give little improvement in the detectable difference of eosinophil percentages. |
| format | Article |
| id | doaj-art-b79c5b7f875c4ec6b1f23b4632372ddc |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b79c5b7f875c4ec6b1f23b4632372ddc2025-02-03T06:05:08ZengWileyMediators of Inflammation0962-93511466-18612002-01-0111529329810.1080/09629350210000015692Induced sputum is a reproducible method to assess airway inflammation in asthmaElena Bacci0Silvana Cianchetti1Stefano Carnevali2Maria Laura Bartoli3Federico L. Dente4Antonella Di Franco5Daniele Giannini6Barbara Vagaggini7Pier Luigi Paggiaro8Cardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyCardiothoracic Department, University of Pisa, Italy U.O. Fisiopatologia Respiratoria, Ospedale di Cisanello, via Paradisa 2, Pisa 56124, ItalyTo evaluate the reproducibility of induced sputum analysis, and to estimate the sample size required to obtained reliable results, sputum was induced by hypertonic saline inhalation in 29 asthmatic subjects on two different days. The whole sample method was used for analysis, and inflammatory cells were counted on cytospin slides. Reproducibility, expressed by intra-class correlation coefficients, was good for macrophages (+0.80), neutrophils (+0.85), and eosinophils (+0.87), but not for lymphocytes (+0.15). Detectable differences were 5.5% for macrophages, 0.6% for lymphocytes, 5.2% for neutrophils, and 3.0% for eosinophils. We conclude that analysis of induced sputum is a reproducible method to study airway inflammation in asthma. Sample sizes greater than ours give little improvement in the detectable difference of eosinophil percentages.http://dx.doi.org/10.1080/09629350210000015692 |
| spellingShingle | Elena Bacci Silvana Cianchetti Stefano Carnevali Maria Laura Bartoli Federico L. Dente Antonella Di Franco Daniele Giannini Barbara Vagaggini Pier Luigi Paggiaro Induced sputum is a reproducible method to assess airway inflammation in asthma Mediators of Inflammation |
| title | Induced sputum is a reproducible method to assess airway inflammation in asthma |
| title_full | Induced sputum is a reproducible method to assess airway inflammation in asthma |
| title_fullStr | Induced sputum is a reproducible method to assess airway inflammation in asthma |
| title_full_unstemmed | Induced sputum is a reproducible method to assess airway inflammation in asthma |
| title_short | Induced sputum is a reproducible method to assess airway inflammation in asthma |
| title_sort | induced sputum is a reproducible method to assess airway inflammation in asthma |
| url | http://dx.doi.org/10.1080/09629350210000015692 |
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