Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen
This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2024-11-01
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| Series: | Ovozoa: Journal of Animal Reproduction |
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| Online Access: | https://e-journal.unair.ac.id/OVZ/article/view/52716 |
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| author | Aditya Irawan Fajar Ramadhan Subiyantoro Suherni Susilowati Tri Wahyu Suprayogi Sri Pantja Madyawati Pudji Srianto Nove Hidajati Eduardus Bimo Aksono Herupradoto Kadek Rachmawati |
| author_facet | Aditya Irawan Fajar Ramadhan Subiyantoro Suherni Susilowati Tri Wahyu Suprayogi Sri Pantja Madyawati Pudji Srianto Nove Hidajati Eduardus Bimo Aksono Herupradoto Kadek Rachmawati |
| author_sort | Aditya Irawan |
| collection | DOAJ |
| description | This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing. |
| format | Article |
| id | doaj-art-b7822f82a8d24c88845bca270f86107d |
| institution | Kabale University |
| issn | 2302-6464 2722-967X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Ovozoa: Journal of Animal Reproduction |
| spelling | doaj-art-b7822f82a8d24c88845bca270f86107d2025-02-06T01:12:29ZengUniversitas AirlanggaOvozoa: Journal of Animal Reproduction2302-64642722-967X2024-11-0113316117010.20473/ovz.v13i3.2024.161-17050876Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semenAditya Irawan0https://orcid.org/0000-0002-8387-4736Fajar Ramadhan Subiyantoro1https://orcid.org/0009-0001-3298-3721Suherni Susilowati2https://orcid.org/0000-0002-2609-088XTri Wahyu Suprayogi3https://orcid.org/0000-0002-2366-5027Sri Pantja Madyawati4https://orcid.org/0000-0002-5864-439XPudji Srianto5https://orcid.org/0000-0002-6832-9799Nove Hidajati6https://orcid.org/0000-0002-8769-5655Eduardus Bimo Aksono Herupradoto7https://orcid.org/0000-0001-6961-2556Kadek Rachmawati8https://orcid.org/0000-0002-0971-3467Internship Veterinarian at Bali Regional Artificial Insemination Center, Tabanan Regency, Bali, IndonesiaInternship Veterinarian at Setia Kawan Dairy Farming Cooperative, Jl. Raya Wonosari Nongkojajar No.38, Tutur District, Pasuruan, IndonesiaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, IndonesiaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, IndonesiaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, IndonesiaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, IndonesiaDivision of Basic Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, IndonesiaDivision of Basic Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, IndonesiaDivision of Basic Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, IndonesiaThis study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.https://e-journal.unair.ac.id/OVZ/article/view/52716antioxidantmorphological abnormalitiesmotilityplasma membrane integrityviability |
| spellingShingle | Aditya Irawan Fajar Ramadhan Subiyantoro Suherni Susilowati Tri Wahyu Suprayogi Sri Pantja Madyawati Pudji Srianto Nove Hidajati Eduardus Bimo Aksono Herupradoto Kadek Rachmawati Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Ovozoa: Journal of Animal Reproduction antioxidant morphological abnormalities motility plasma membrane integrity viability |
| title | Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen |
| title_full | Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen |
| title_fullStr | Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen |
| title_full_unstemmed | Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen |
| title_short | Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen |
| title_sort | addition of basil leaf ocimum basicilum l extract to andromed r extender improved the quality of spermatozoa of frozen thawed simmental bull semen |
| topic | antioxidant morphological abnormalities motility plasma membrane integrity viability |
| url | https://e-journal.unair.ac.id/OVZ/article/view/52716 |
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