Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae)
<i>Encyclia</i> Hook. is a tropical orchid known for its delightful fragrance and distinctive flower shapes, which have been widely used as superior parents for inter and intrageneric breeding. In this study, karyotype analysis shows that the number of chromosomes in diploid plants of &l...
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2025-01-01
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| author | Chengcheng Zhou Qingyun Leng Jinping Lu Shisong Xu Donghui Peng Junmei Yin Junhai Niu |
| author_facet | Chengcheng Zhou Qingyun Leng Jinping Lu Shisong Xu Donghui Peng Junmei Yin Junhai Niu |
| author_sort | Chengcheng Zhou |
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| description | <i>Encyclia</i> Hook. is a tropical orchid known for its delightful fragrance and distinctive flower shapes, which have been widely used as superior parents for inter and intrageneric breeding. In this study, karyotype analysis shows that the number of chromosomes in diploid plants of <i>Encyclia</i> is 40, with all species exhibiting mesial and central filamentous chromosomes. The karyotype of diploid plants is primarily 2B. Among the three detected intergeneric hybrids, both <i>Enanthleya</i> Circus Lady ‘Coastal Star’ and <i>Guaricyclia</i> Kyoguchi ‘Fumi’ are triploid, while <i>Robertsara</i> ‘Green Wonder’ is tetraploid. By evaluating various lysates and tissues, a flow cytometry system (FCM) is developed with Galbraith’s buffer (GLB) and tender leaves. By using <i>Dendrobium officinale</i> as an external reference, the genome size of the <i>Encyclia</i> species and its intra and intergeneric hybrids are assessed, which ranges from 1.07 Gb to 3.23 Gb. These results will establish a foundation for high-throughput identification of germplasms, advance crossbreeding efforts, and enhance the understanding of the genome in <i>Encyclia</i>. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-b6f92e7d41c24db99ea986d7a46164c22025-01-24T13:29:30ZengMDPI AGDiversity1424-28182025-01-011715710.3390/d17010057Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae)Chengcheng Zhou0Qingyun Leng1Jinping Lu2Shisong Xu3Donghui Peng4Junmei Yin5Junhai Niu6College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Key Laboratory for Tropical Crop Breeding, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571737, ChinaNational Key Laboratory for Tropical Crop Breeding, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571737, ChinaNational Key Laboratory for Tropical Crop Breeding, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571737, ChinaCollege of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaNational Key Laboratory for Tropical Crop Breeding, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571737, ChinaNational Key Laboratory for Tropical Crop Breeding, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571737, China<i>Encyclia</i> Hook. is a tropical orchid known for its delightful fragrance and distinctive flower shapes, which have been widely used as superior parents for inter and intrageneric breeding. In this study, karyotype analysis shows that the number of chromosomes in diploid plants of <i>Encyclia</i> is 40, with all species exhibiting mesial and central filamentous chromosomes. The karyotype of diploid plants is primarily 2B. Among the three detected intergeneric hybrids, both <i>Enanthleya</i> Circus Lady ‘Coastal Star’ and <i>Guaricyclia</i> Kyoguchi ‘Fumi’ are triploid, while <i>Robertsara</i> ‘Green Wonder’ is tetraploid. By evaluating various lysates and tissues, a flow cytometry system (FCM) is developed with Galbraith’s buffer (GLB) and tender leaves. By using <i>Dendrobium officinale</i> as an external reference, the genome size of the <i>Encyclia</i> species and its intra and intergeneric hybrids are assessed, which ranges from 1.07 Gb to 3.23 Gb. These results will establish a foundation for high-throughput identification of germplasms, advance crossbreeding efforts, and enhance the understanding of the genome in <i>Encyclia</i>.https://www.mdpi.com/1424-2818/17/1/57<i>Encyclia</i>karyotypeflow cytometrygenome size |
| spellingShingle | Chengcheng Zhou Qingyun Leng Jinping Lu Shisong Xu Donghui Peng Junmei Yin Junhai Niu Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae) Diversity <i>Encyclia</i> karyotype flow cytometry genome size |
| title | Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae) |
| title_full | Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae) |
| title_fullStr | Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae) |
| title_full_unstemmed | Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae) |
| title_short | Karyotype Analysis and Genome Size Estimation Using Flow Cytometry of the Genus <i>Encyclia</i> Hook. (Orchidaceae: Laeliinae) |
| title_sort | karyotype analysis and genome size estimation using flow cytometry of the genus i encyclia i hook orchidaceae laeliinae |
| topic | <i>Encyclia</i> karyotype flow cytometry genome size |
| url | https://www.mdpi.com/1424-2818/17/1/57 |
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