Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats
Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-β-cyclodextrin (HP-β-CD) and formulated with Eudragit E100, glycero...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2016-0018 |
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| _version_ | 1832572156100214784 |
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| author | Sawatdee Somchai Choochuay Kanuengnit Chanthorn Wirot Srichana Teerapol |
| author_facet | Sawatdee Somchai Choochuay Kanuengnit Chanthorn Wirot Srichana Teerapol |
| author_sort | Sawatdee Somchai |
| collection | DOAJ |
| description | Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-β-cyclodextrin (HP-β-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.5–6.0 with viscosity in the range of 20–60 mPa s, surface tension 20.3–24.6 mN m–1 and contact angle less than 20°. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An in vivo excision wound healing model showed that wound excision was completely healed after 14 days. |
| format | Article |
| id | doaj-art-b6d06259c15b46579cbd89db68d128e9 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2016-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-b6d06259c15b46579cbd89db68d128e92025-02-02T11:49:55ZengSciendoActa Pharmaceutica1846-95582016-06-0166223324410.1515/acph-2016-0018acph-2016-0018Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in ratsSawatdee SomchaiChoochuay KanuengnitChanthorn Wirot0Srichana Teerapol1 Faculty of Public Health, Naresuan University, Tha Pho, Muang, Phitsanulok, 65000, Thailand Department of Pharmaceutical, Technology and Nanotec-PSU, Excellence Center on Drug Delivery System, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandCentella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-β-cyclodextrin (HP-β-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.5–6.0 with viscosity in the range of 20–60 mPa s, surface tension 20.3–24.6 mN m–1 and contact angle less than 20°. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An in vivo excision wound healing model showed that wound excision was completely healed after 14 days.https://doi.org/10.1515/acph-2016-0018centella asiatica extracthydroxypropyl-β-cyclodextrinwound healingtopical spray |
| spellingShingle | Sawatdee Somchai Choochuay Kanuengnit Chanthorn Wirot Srichana Teerapol Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats Acta Pharmaceutica centella asiatica extract hydroxypropyl-β-cyclodextrin wound healing topical spray |
| title | Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats |
| title_full | Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats |
| title_fullStr | Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats |
| title_full_unstemmed | Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats |
| title_short | Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats |
| title_sort | evaluation of the topical spray containing centella asiatica extract and its efficacy on excision wounds in rats |
| topic | centella asiatica extract hydroxypropyl-β-cyclodextrin wound healing topical spray |
| url | https://doi.org/10.1515/acph-2016-0018 |
| work_keys_str_mv | AT sawatdeesomchai evaluationofthetopicalspraycontainingcentellaasiaticaextractanditsefficacyonexcisionwoundsinrats AT choochuaykanuengnit evaluationofthetopicalspraycontainingcentellaasiaticaextractanditsefficacyonexcisionwoundsinrats AT chanthornwirot evaluationofthetopicalspraycontainingcentellaasiaticaextractanditsefficacyonexcisionwoundsinrats AT srichanateerapol evaluationofthetopicalspraycontainingcentellaasiaticaextractanditsefficacyonexcisionwoundsinrats |