Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspring

Abstract Cannabis use in pregnancy is associated with low birthweight outcomes. Recent preclinical data suggests that maternal Δ9-tetrahydrocannabinol (THC) exposure leads to decreases in birthweight followed by early cardiac deficits in offspring. Currently, no studies have explored an intervention...

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Main Authors: Kendrick Lee, Mohammed H. Sarikahya, Samantha L. Cousineau, Ken K.-C. Yeung, Amica Lucas, Kara Loudon, Thane Tomy, Gregg T. Tomy, David R. C. Natale, Steven R. Laviolette, Daniel B. Hardy
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Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-92844-6
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author Kendrick Lee
Mohammed H. Sarikahya
Samantha L. Cousineau
Ken K.-C. Yeung
Amica Lucas
Kara Loudon
Thane Tomy
Gregg T. Tomy
David R. C. Natale
Steven R. Laviolette
Daniel B. Hardy
author_facet Kendrick Lee
Mohammed H. Sarikahya
Samantha L. Cousineau
Ken K.-C. Yeung
Amica Lucas
Kara Loudon
Thane Tomy
Gregg T. Tomy
David R. C. Natale
Steven R. Laviolette
Daniel B. Hardy
author_sort Kendrick Lee
collection DOAJ
description Abstract Cannabis use in pregnancy is associated with low birthweight outcomes. Recent preclinical data suggests that maternal Δ9-tetrahydrocannabinol (THC) exposure leads to decreases in birthweight followed by early cardiac deficits in offspring. Currently, no studies have explored an intervention for these maternal THC-induced deficits. Omega-3 fatty acids have been shown to exhibit cardioprotective effects. In this present study, we demonstrated that maternal dietary supplementation of omega-3 fatty acids ameliorates both THC-induced fetal growth and postnatal cardiac deficits in offspring. Our data indicates this may be underpinned by alterations in cardiac and hepatic fatty acids and reduction in markers of cardiac collagen deposition. Interestingly, the cardioprotective effects of omega-3s may be further underscored by decreased signaling of the cardiac endocannabinoid system. With increasing rates of cannabis use in pregnancy and recent evidence of subsequent cardiometabolic aberrations in offspring, our data suggests a potential intervention for THC-induced fetal growth and cardiac disturbances in offspring.
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spelling doaj-art-b6a9bd9454734bf6b32b723e2da98ec02025-08-20T02:56:06ZengNature PortfolioScientific Reports2045-23222025-03-0115111510.1038/s41598-025-92844-6Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspringKendrick Lee0Mohammed H. Sarikahya1Samantha L. Cousineau2Ken K.-C. Yeung3Amica Lucas4Kara Loudon5Thane Tomy6Gregg T. Tomy7David R. C. Natale8Steven R. Laviolette9Daniel B. Hardy10Department of Physiology and Pharmacology, Western UniversityDepartment of Anatomy and Cell Biology, Schulich School of Medicine and DentistryWestern UniversityDepartment of Chemistry and Biochemistry, Western UniversityDepartment of Chemistry and Biochemistry, Western UniversityUniversity of ManitobaUniversity of ManitobaUniversity of ManitobaUniversity of ManitobaQueen’s UniversityDepartment of Anatomy and Cell Biology, Schulich School of Medicine and DentistryWestern UniversityDepartment of Physiology and Pharmacology, Western UniversityAbstract Cannabis use in pregnancy is associated with low birthweight outcomes. Recent preclinical data suggests that maternal Δ9-tetrahydrocannabinol (THC) exposure leads to decreases in birthweight followed by early cardiac deficits in offspring. Currently, no studies have explored an intervention for these maternal THC-induced deficits. Omega-3 fatty acids have been shown to exhibit cardioprotective effects. In this present study, we demonstrated that maternal dietary supplementation of omega-3 fatty acids ameliorates both THC-induced fetal growth and postnatal cardiac deficits in offspring. Our data indicates this may be underpinned by alterations in cardiac and hepatic fatty acids and reduction in markers of cardiac collagen deposition. Interestingly, the cardioprotective effects of omega-3s may be further underscored by decreased signaling of the cardiac endocannabinoid system. With increasing rates of cannabis use in pregnancy and recent evidence of subsequent cardiometabolic aberrations in offspring, our data suggests a potential intervention for THC-induced fetal growth and cardiac disturbances in offspring.https://doi.org/10.1038/s41598-025-92844-6CannabisMaternalOmega-3 fatty acidsCardiacΔ9-tetrahydrocannabinol (THC)Endocannabinoid system
spellingShingle Kendrick Lee
Mohammed H. Sarikahya
Samantha L. Cousineau
Ken K.-C. Yeung
Amica Lucas
Kara Loudon
Thane Tomy
Gregg T. Tomy
David R. C. Natale
Steven R. Laviolette
Daniel B. Hardy
Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspring
Scientific Reports
Cannabis
Maternal
Omega-3 fatty acids
Cardiac
Δ9-tetrahydrocannabinol (THC)
Endocannabinoid system
title Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspring
title_full Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspring
title_fullStr Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspring
title_full_unstemmed Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspring
title_short Maternal dietary DHA and EPA supplementation ameliorates adverse cardiac outcomes in THC-exposed rat offspring
title_sort maternal dietary dha and epa supplementation ameliorates adverse cardiac outcomes in thc exposed rat offspring
topic Cannabis
Maternal
Omega-3 fatty acids
Cardiac
Δ9-tetrahydrocannabinol (THC)
Endocannabinoid system
url https://doi.org/10.1038/s41598-025-92844-6
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