REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus
Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identificat...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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Series: | Вавиловский журнал генетики и селекции |
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Online Access: | https://vavilov.elpub.ru/jour/article/view/198 |
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author | K. V. Starostin E. A. Demidov A. S. Rozanov A. V. Bryanskaya S. E. Peltek |
author_facet | K. V. Starostin E. A. Demidov A. S. Rozanov A. V. Bryanskaya S. E. Peltek |
author_sort | K. V. Starostin |
collection | DOAJ |
description | Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains. |
format | Article |
id | doaj-art-b5f1fc5e4d934c5f8e551eb6d24c6151 |
institution | Kabale University |
issn | 2500-3259 |
language | English |
publishDate | 2015-01-01 |
publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
record_format | Article |
series | Вавиловский журнал генетики и селекции |
spelling | doaj-art-b5f1fc5e4d934c5f8e551eb6d24c61512025-02-01T09:58:00ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01174/1748757182REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilusK. V. Starostin0E. A. Demidov1A. S. Rozanov2A. V. Bryanskaya3S. E. Peltek4Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaRapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains.https://vavilov.elpub.ru/jour/article/view/198maldidirect protein profilingmicroorganism identificationgeobacillus stearothermоphilus |
spellingShingle | K. V. Starostin E. A. Demidov A. S. Rozanov A. V. Bryanskaya S. E. Peltek REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus Вавиловский журнал генетики и селекции maldi direct protein profiling microorganism identification geobacillus stearothermоphilus |
title | REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus |
title_full | REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus |
title_fullStr | REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus |
title_full_unstemmed | REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus |
title_short | REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus |
title_sort | reproducibility of the results of microbe identification by maldi tof mass spectrometry depending on growth conditions by the example of geobacillus stearothermоphilus |
topic | maldi direct protein profiling microorganism identification geobacillus stearothermоphilus |
url | https://vavilov.elpub.ru/jour/article/view/198 |
work_keys_str_mv | AT kvstarostin reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus AT eademidov reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus AT asrozanov reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus AT avbryanskaya reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus AT sepeltek reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus |