REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus

Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identificat...

Full description

Saved in:
Bibliographic Details
Main Authors: K. V. Starostin, E. A. Demidov, A. S. Rozanov, A. V. Bryanskaya, S. E. Peltek
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2015-01-01
Series:Вавиловский журнал генетики и селекции
Subjects:
Online Access:https://vavilov.elpub.ru/jour/article/view/198
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832575324772106240
author K. V. Starostin
E. A. Demidov
A. S. Rozanov
A. V. Bryanskaya
S. E. Peltek
author_facet K. V. Starostin
E. A. Demidov
A. S. Rozanov
A. V. Bryanskaya
S. E. Peltek
author_sort K. V. Starostin
collection DOAJ
description Rapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains.
format Article
id doaj-art-b5f1fc5e4d934c5f8e551eb6d24c6151
institution Kabale University
issn 2500-3259
language English
publishDate 2015-01-01
publisher Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
record_format Article
series Вавиловский журнал генетики и селекции
spelling doaj-art-b5f1fc5e4d934c5f8e551eb6d24c61512025-02-01T09:58:00ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01174/1748757182REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilusK. V. Starostin0E. A. Demidov1A. S. Rozanov2A. V. Bryanskaya3S. E. Peltek4Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaRapid and accurate methods of microorganism identification are essential in various human activities. They include primarily clinical diagnostics. In addition, they are in demand in environment monitoring, harmacology, food industry, research, etc. There are diverse approaches to microbe identification: phenotyping, genotyping, chemotaxonomy, direct protein profiling, etc. In this work, the effects of various growth conditions, such as temperature, growth time, and nutrition medium, on the reproducibility of microbe identification by MALDI-TOF mass spectrometry are considered by the example of three Geobacillus stearothermоphilus strains.https://vavilov.elpub.ru/jour/article/view/198maldidirect protein profilingmicroorganism identificationgeobacillus stearothermоphilus
spellingShingle K. V. Starostin
E. A. Demidov
A. S. Rozanov
A. V. Bryanskaya
S. E. Peltek
REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus
Вавиловский журнал генетики и селекции
maldi
direct protein profiling
microorganism identification
geobacillus stearothermоphilus
title REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus
title_full REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus
title_fullStr REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus
title_full_unstemmed REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus
title_short REPRODUCIBILITY OF THE RESULTS OF MICROBE IDENTIFICATION BY MALDI-TOF MASS SPECTROMETRY DEPENDING ON GROWTH CONDITIONS BY THE EXAMPLE OF Geobacillus stearothermоphilus
title_sort reproducibility of the results of microbe identification by maldi tof mass spectrometry depending on growth conditions by the example of geobacillus stearothermоphilus
topic maldi
direct protein profiling
microorganism identification
geobacillus stearothermоphilus
url https://vavilov.elpub.ru/jour/article/view/198
work_keys_str_mv AT kvstarostin reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus
AT eademidov reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus
AT asrozanov reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus
AT avbryanskaya reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus
AT sepeltek reproducibilityoftheresultsofmicrobeidentificationbymalditofmassspectrometrydependingongrowthconditionsbytheexampleofgeobacillusstearothermophilus