Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants
Background. Mucosal or oral tolerance, an established method for inducing low-risk antigen-specific hyporesponsiveness, has not been investigated in vascularized composite allograft (VCA) research. We studied its effects on recipient immune responses and VCA rejection. Methods. Lewis rats (; TREATED...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/704063 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564008209612800 |
|---|---|
| author | Christopher Glenn Wallace Chia-Hung Yen Hsiang-Chen Yang Chun-Yen Lin Ren-Chin Wu Wei-Chao Huang Jeng-Yee Lin Fu-Chan Wei |
| author_facet | Christopher Glenn Wallace Chia-Hung Yen Hsiang-Chen Yang Chun-Yen Lin Ren-Chin Wu Wei-Chao Huang Jeng-Yee Lin Fu-Chan Wei |
| author_sort | Christopher Glenn Wallace |
| collection | DOAJ |
| description | Background. Mucosal or oral tolerance, an established method for inducing low-risk antigen-specific hyporesponsiveness, has not been investigated in vascularized composite allograft (VCA) research. We studied its effects on recipient immune responses and VCA rejection. Methods. Lewis rats (; TREATED) received seven daily intrajejunal treatments of splenocytes from semiallogeneic Lewis-Brown-Norway rats (LBN) or vehicle (; SHAM). Recipients’ immune responses were assessed by mixed lymphocyte reaction (MLR) against donor antigen and controls. Other Lewis (; TREATED/VCA) received LBN hindlimb VCA and daily intrajejunal treatments of LBN splenocytes, or LBN VCA without treatment (; SHAM/VCA), until VCAs rejected. Recipients’ immune responses were characterised and VCAs biopsied for histopathology. Immunosuppressants were not used.
Results. LBN-specific hyporesponsiveness was induced only in treated Lewis recipients. Treatment significantly reduced MLR alloreactivity, significantly reduced VCA rejection on histopathology, and significantly delayed clinical VCA rejection (; TREATED/VCA mean 9.6 versus 6.0 days for SHAM/VCA). Treatment significantly increased immunosuppressive IL-10/IL-4/TGF-β production and significantly decreased proinflammatory IFN-γ/TNF-α. Conclusion. Jejunal exposure to antigen conferred donor specific hyporesponsiveness that delayed VCA rejection. This method may offer a low-risk adjunctive treatment option to help protect VCAs from rejection. |
| format | Article |
| id | doaj-art-b56a3a86366440ca9d989fda1b0fc4b9 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-b56a3a86366440ca9d989fda1b0fc4b92025-02-03T01:12:04ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/704063704063Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant ImmunosuppressantsChristopher Glenn Wallace0Chia-Hung Yen1Hsiang-Chen Yang2Chun-Yen Lin3Ren-Chin Wu4Wei-Chao Huang5Jeng-Yee Lin6Fu-Chan Wei7Department of Plastic Surgery, Chang Gung Memorial Hospital, Chang Gung University and Medical College, 199 Tun Hwa North Road, Taipei 105, TaiwanDepartment of Biological Science and Technology, National Pingtung University of Science and Technology 1 Hseuh-Fu Road, Neipu 91201, Pingtung, TaiwanDepartment of Plastic Surgery, Chang Gung Memorial Hospital, Chang Gung University and Medical College, 199 Tun Hwa North Road, Taipei 105, TaiwanDepartment of Hepatogastroenterology, Chang Gung Memorial Hospital, Chang Gung University and Medical College, 199 Tun Hwa North Road, Taipei 105, TaiwanDepartment of Pathology, Chang Gung Memorial Hospital, Chang Gung University and Medical College, 199 Tun Hwa North Road, Taipei 105, TaiwanDepartment of Plastic Surgery, Chang Gung Memorial Hospital, Chia-Yi, TaiwanDepartment of Plastic Surgery, Chang Gung Memorial Hospital, Chang Gung University and Medical College, 199 Tun Hwa North Road, Taipei 105, TaiwanDepartment of Plastic Surgery, Chang Gung Memorial Hospital, Chang Gung University and Medical College, 199 Tun Hwa North Road, Taipei 105, TaiwanBackground. Mucosal or oral tolerance, an established method for inducing low-risk antigen-specific hyporesponsiveness, has not been investigated in vascularized composite allograft (VCA) research. We studied its effects on recipient immune responses and VCA rejection. Methods. Lewis rats (; TREATED) received seven daily intrajejunal treatments of splenocytes from semiallogeneic Lewis-Brown-Norway rats (LBN) or vehicle (; SHAM). Recipients’ immune responses were assessed by mixed lymphocyte reaction (MLR) against donor antigen and controls. Other Lewis (; TREATED/VCA) received LBN hindlimb VCA and daily intrajejunal treatments of LBN splenocytes, or LBN VCA without treatment (; SHAM/VCA), until VCAs rejected. Recipients’ immune responses were characterised and VCAs biopsied for histopathology. Immunosuppressants were not used. Results. LBN-specific hyporesponsiveness was induced only in treated Lewis recipients. Treatment significantly reduced MLR alloreactivity, significantly reduced VCA rejection on histopathology, and significantly delayed clinical VCA rejection (; TREATED/VCA mean 9.6 versus 6.0 days for SHAM/VCA). Treatment significantly increased immunosuppressive IL-10/IL-4/TGF-β production and significantly decreased proinflammatory IFN-γ/TNF-α. Conclusion. Jejunal exposure to antigen conferred donor specific hyporesponsiveness that delayed VCA rejection. This method may offer a low-risk adjunctive treatment option to help protect VCAs from rejection.http://dx.doi.org/10.1155/2012/704063 |
| spellingShingle | Christopher Glenn Wallace Chia-Hung Yen Hsiang-Chen Yang Chun-Yen Lin Ren-Chin Wu Wei-Chao Huang Jeng-Yee Lin Fu-Chan Wei Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants Clinical and Developmental Immunology |
| title | Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants |
| title_full | Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants |
| title_fullStr | Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants |
| title_full_unstemmed | Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants |
| title_short | Vascularized Composite Allograft Rejection Is Delayed by Intrajejunal Treatment with Donor Splenocytes without Concomitant Immunosuppressants |
| title_sort | vascularized composite allograft rejection is delayed by intrajejunal treatment with donor splenocytes without concomitant immunosuppressants |
| url | http://dx.doi.org/10.1155/2012/704063 |
| work_keys_str_mv | AT christopherglennwallace vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants AT chiahungyen vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants AT hsiangchenyang vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants AT chunyenlin vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants AT renchinwu vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants AT weichaohuang vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants AT jengyeelin vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants AT fuchanwei vascularizedcompositeallograftrejectionisdelayedbyintrajejunaltreatmentwithdonorsplenocyteswithoutconcomitantimmunosuppressants |