Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel Explants
Polygalacturonase (PG) is crucial in plant organ abscission process. This paper investigated the cellular and subcellular localization of PG in ethylene-stimulated abscission of tomato pedicel explants. Confocal laser scanning microscopy of abscission zone sections with the fluorescent probe Cy3 rev...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/389896 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558059880185856 |
|---|---|
| author | Ming-Fang Qi Tao Xu Wei-Zhi Chen Tian-lai Li |
| author_facet | Ming-Fang Qi Tao Xu Wei-Zhi Chen Tian-lai Li |
| author_sort | Ming-Fang Qi |
| collection | DOAJ |
| description | Polygalacturonase (PG) is crucial in plant organ abscission process. This paper investigated the cellular and subcellular localization of PG in ethylene-stimulated abscission of tomato pedicel explants. Confocal laser scanning microscopy of abscission zone sections with the fluorescent probe Cy3 revealed that PG was initially accumulated in parenchyma cells in cortical and vascular tissues after 8 h of ethylene treatment and then extended throughout the abscission zone when the abscission zone separated at 24 h after ethylene treatment. At the subcellular level, transmission electron microscopy with immunogold staining showed that PG showed abundant accumulation in the cortical and vascular tissues at 8 h after ethylene treatment, and the distribution area extended to the central parenchyma cells at 16 h after ethylene treatment. In addition, PGs were observed in the distal and proximal parts of the tomato pedicel explants throughout the abscission process. The results provided a visualized distribution of PG in the pedicel abscission zone and proved that PG was closely related to abscission. |
| format | Article |
| id | doaj-art-b55a37e1d5204e19a447979ac5c7274a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b55a37e1d5204e19a447979ac5c7274a2025-02-03T01:33:25ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/389896389896Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel ExplantsMing-Fang Qi0Tao Xu1Wei-Zhi Chen2Tian-lai Li3Department of Horticulture, Key Laboratory of Protected Horticulture, Ministry of Education, No. 120 Donglin Road, Shenyang City, Liaoning 110866, ChinaDepartment of Horticulture, Key Laboratory of Protected Horticulture, Ministry of Education, No. 120 Donglin Road, Shenyang City, Liaoning 110866, ChinaDepartment of Horticulture, Key Laboratory of Protected Horticulture, Ministry of Education, No. 120 Donglin Road, Shenyang City, Liaoning 110866, ChinaDepartment of Horticulture, Key Laboratory of Protected Horticulture, Ministry of Education, No. 120 Donglin Road, Shenyang City, Liaoning 110866, ChinaPolygalacturonase (PG) is crucial in plant organ abscission process. This paper investigated the cellular and subcellular localization of PG in ethylene-stimulated abscission of tomato pedicel explants. Confocal laser scanning microscopy of abscission zone sections with the fluorescent probe Cy3 revealed that PG was initially accumulated in parenchyma cells in cortical and vascular tissues after 8 h of ethylene treatment and then extended throughout the abscission zone when the abscission zone separated at 24 h after ethylene treatment. At the subcellular level, transmission electron microscopy with immunogold staining showed that PG showed abundant accumulation in the cortical and vascular tissues at 8 h after ethylene treatment, and the distribution area extended to the central parenchyma cells at 16 h after ethylene treatment. In addition, PGs were observed in the distal and proximal parts of the tomato pedicel explants throughout the abscission process. The results provided a visualized distribution of PG in the pedicel abscission zone and proved that PG was closely related to abscission.http://dx.doi.org/10.1155/2014/389896 |
| spellingShingle | Ming-Fang Qi Tao Xu Wei-Zhi Chen Tian-lai Li Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel Explants The Scientific World Journal |
| title | Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel Explants |
| title_full | Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel Explants |
| title_fullStr | Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel Explants |
| title_full_unstemmed | Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel Explants |
| title_short | Ultrastructural Localization of Polygalacturonase in Ethylene-Stimulated Abscission of Tomato Pedicel Explants |
| title_sort | ultrastructural localization of polygalacturonase in ethylene stimulated abscission of tomato pedicel explants |
| url | http://dx.doi.org/10.1155/2014/389896 |
| work_keys_str_mv | AT mingfangqi ultrastructurallocalizationofpolygalacturonaseinethylenestimulatedabscissionoftomatopedicelexplants AT taoxu ultrastructurallocalizationofpolygalacturonaseinethylenestimulatedabscissionoftomatopedicelexplants AT weizhichen ultrastructurallocalizationofpolygalacturonaseinethylenestimulatedabscissionoftomatopedicelexplants AT tianlaili ultrastructurallocalizationofpolygalacturonaseinethylenestimulatedabscissionoftomatopedicelexplants |