Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B
Recombinant Bacillus Calmette-Guérin (rBCG) strain is the promising vaccine candidate for tuberculosis (TB) prevention, which aims at providing more enduring and enhanced protection than the parental BCG vaccine. In this study, three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::8...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/563838 |
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| author | Chun Wang Ruiling Fu Zhenhua Chen Kun Tan Lingxia Chen Xindong Teng Jia Lu Chunwei Shi Xionglin Fan |
| author_facet | Chun Wang Ruiling Fu Zhenhua Chen Kun Tan Lingxia Chen Xindong Teng Jia Lu Chunwei Shi Xionglin Fan |
| author_sort | Chun Wang |
| collection | DOAJ |
| description | Recombinant Bacillus Calmette-Guérin (rBCG) strain is the promising vaccine candidate for tuberculosis (TB) prevention, which aims at providing more enduring and enhanced protection than the parental BCG vaccine. In this study, three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed, respectively. rBCG strains showed higher level of overexpression of Ag85A and/or Ag85B proteins than BCG containing empty vector pMV261(rBCG::261), which had low levels of endogenous expression of both proteins as expected. rBCG::AB strain could provide the strongest short-term and long-term protection in the lung against intravenous infection with virulent M. tuberculosis than rBCG::261 control and other two rBCG strains overexpressing single antigen. The stronger and longer-lasting protection provided by rBCG::AB than rBCG::261 was correlated with systemic in vitro antigen-specific IFN-γ responses. Therefore, our results indicate that rBCG::AB could be a very promising TB vaccine candidate and should be further evaluated for the preclinical test. |
| format | Article |
| id | doaj-art-b4801bcfa968448c87e42e4614ad99b7 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-b4801bcfa968448c87e42e4614ad99b72025-02-03T01:00:59ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/563838563838Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85BChun Wang0Ruiling Fu1Zhenhua Chen2Kun Tan3Lingxia Chen4Xindong Teng5Jia Lu6Chunwei Shi7Xionglin Fan8Laboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaLaboratory of Biosafety, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science & Technology, No. 13, Hangkong Road, Wuhan 430030, ChinaRecombinant Bacillus Calmette-Guérin (rBCG) strain is the promising vaccine candidate for tuberculosis (TB) prevention, which aims at providing more enduring and enhanced protection than the parental BCG vaccine. In this study, three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed, respectively. rBCG strains showed higher level of overexpression of Ag85A and/or Ag85B proteins than BCG containing empty vector pMV261(rBCG::261), which had low levels of endogenous expression of both proteins as expected. rBCG::AB strain could provide the strongest short-term and long-term protection in the lung against intravenous infection with virulent M. tuberculosis than rBCG::261 control and other two rBCG strains overexpressing single antigen. The stronger and longer-lasting protection provided by rBCG::AB than rBCG::261 was correlated with systemic in vitro antigen-specific IFN-γ responses. Therefore, our results indicate that rBCG::AB could be a very promising TB vaccine candidate and should be further evaluated for the preclinical test.http://dx.doi.org/10.1155/2012/563838 |
| spellingShingle | Chun Wang Ruiling Fu Zhenhua Chen Kun Tan Lingxia Chen Xindong Teng Jia Lu Chunwei Shi Xionglin Fan Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B Clinical and Developmental Immunology |
| title | Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B |
| title_full | Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B |
| title_fullStr | Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B |
| title_full_unstemmed | Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B |
| title_short | Immunogenicity and Protective Efficacy of a Novel Recombinant BCG Strain Overexpressing Antigens Ag85A and Ag85B |
| title_sort | immunogenicity and protective efficacy of a novel recombinant bcg strain overexpressing antigens ag85a and ag85b |
| url | http://dx.doi.org/10.1155/2012/563838 |
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