17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells.
17β-estradiol (E2) has been shown to have beneficial effects on the cardiovascular system. We previously demonstrated that E2 increases striatin levels and inhibits migration in vascular smooth muscle cells. The objective of the present study was to investigate the effects of E2 on the regulation of...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0202500&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592740802625536 |
|---|---|
| author | Shuhui Zheng Peng Sun Haimei Liu Runmei Li Lingli Long Yuxia Xu Suiqing Chen Jinwen Xu |
| author_facet | Shuhui Zheng Peng Sun Haimei Liu Runmei Li Lingli Long Yuxia Xu Suiqing Chen Jinwen Xu |
| author_sort | Shuhui Zheng |
| collection | DOAJ |
| description | 17β-estradiol (E2) has been shown to have beneficial effects on the cardiovascular system. We previously demonstrated that E2 increases striatin levels and inhibits migration in vascular smooth muscle cells. The objective of the present study was to investigate the effects of E2 on the regulation of striatin expression in human umbilical vein endothelial cells (HUVECs). We demonstrated that E2 increased striatin protein expression in a dose- and time-dependent manner in HUVECs. Pretreatment with ICI 182780 or the phosphatidylinositol-3 kinase inhibitor, wortmannin, abolished E2-mediated upregulation of striatin protein expression. Treatment with E2 resulted in Akt phosphorylation in a time-dependent manner. Moreover, silencing striatin significantly inhibited HUVEC migration, while striatin overexpression significantly promoted HUVEC migration. Finally, E2 enhanced HUVEC migration, which was inhibited by silencing striatin. In conclusion, our results demonstrated that E2-mediated upregulation of striatin promotes cell migration in HUVECs. |
| format | Article |
| id | doaj-art-b43c91cc9bca4fed934fce3b08d2f4ba |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b43c91cc9bca4fed934fce3b08d2f4ba2025-01-21T05:31:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020250010.1371/journal.pone.020250017β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells.Shuhui ZhengPeng SunHaimei LiuRunmei LiLingli LongYuxia XuSuiqing ChenJinwen Xu17β-estradiol (E2) has been shown to have beneficial effects on the cardiovascular system. We previously demonstrated that E2 increases striatin levels and inhibits migration in vascular smooth muscle cells. The objective of the present study was to investigate the effects of E2 on the regulation of striatin expression in human umbilical vein endothelial cells (HUVECs). We demonstrated that E2 increased striatin protein expression in a dose- and time-dependent manner in HUVECs. Pretreatment with ICI 182780 or the phosphatidylinositol-3 kinase inhibitor, wortmannin, abolished E2-mediated upregulation of striatin protein expression. Treatment with E2 resulted in Akt phosphorylation in a time-dependent manner. Moreover, silencing striatin significantly inhibited HUVEC migration, while striatin overexpression significantly promoted HUVEC migration. Finally, E2 enhanced HUVEC migration, which was inhibited by silencing striatin. In conclusion, our results demonstrated that E2-mediated upregulation of striatin promotes cell migration in HUVECs.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0202500&type=printable |
| spellingShingle | Shuhui Zheng Peng Sun Haimei Liu Runmei Li Lingli Long Yuxia Xu Suiqing Chen Jinwen Xu 17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. PLoS ONE |
| title | 17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. |
| title_full | 17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. |
| title_fullStr | 17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. |
| title_full_unstemmed | 17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. |
| title_short | 17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. |
| title_sort | 17β estradiol upregulates striatin protein levels via akt pathway in human umbilical vein endothelial cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0202500&type=printable |
| work_keys_str_mv | AT shuhuizheng 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells AT pengsun 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells AT haimeiliu 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells AT runmeili 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells AT linglilong 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells AT yuxiaxu 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells AT suiqingchen 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells AT jinwenxu 17bestradiolupregulatesstriatinproteinlevelsviaaktpathwayinhumanumbilicalveinendothelialcells |