Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss)
This experiment aimed to evaluate the effects of triploidization and culture densities on the performance of rainbow trout. Juveniles (30.70 ± 1.04 g) were randomly stocked into 24 fiberglass tanks with a flow-through system. The experiment was designed with two ploidy conditions (diploid and triplo...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006288 |
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| author | Roghayeh Mahmoudi Mahdi Naderi Hakimeh Dopeikar Seyed Ali Khorasaninasab Seyed Hadi Seyedalhosseini |
| author_facet | Roghayeh Mahmoudi Mahdi Naderi Hakimeh Dopeikar Seyed Ali Khorasaninasab Seyed Hadi Seyedalhosseini |
| author_sort | Roghayeh Mahmoudi |
| collection | DOAJ |
| description | This experiment aimed to evaluate the effects of triploidization and culture densities on the performance of rainbow trout. Juveniles (30.70 ± 1.04 g) were randomly stocked into 24 fiberglass tanks with a flow-through system. The experiment was designed with two ploidy conditions (diploid and triploid) each with 20 (40 fish/tank), 30 (59 fish/tank), 40 (79 fish/tank), and 50 (99 fish/tank) kg/m3 culture densities, a 2 × 4 factorial design. After 9 weeks, final weight, weight gain, specific growth rate, and protein efficiency ratio (PER) in fish cultured at 20, 30, and 40 kg/m3 were significantly higher and feed conversion ratio (FCR) was lower compared to those cultured at 50 kg/m3 (P < 0.05). Also, at 50 kg/m3 density, FCR in triploids was significantly higher and PER was lower compared to diploids (P < 0.05). Serum cortisol levels were highest and osmolality and total antioxidant capacity values were lowest in triploid fish cultured at the highest (50 kg/m3) density, indicating that triploids were affected more than diploids by chronic high density stress. Serum lysozyme and alternative complement (ACH50) activities and globulin content were significantly lower in fish cultured at 50 kg/m3 (P < 0.05). Thus, rainbow trout chronically exposed to high culture density (50 kg/m3) showed signs of immunosuppression. Chronic high density stress exhibited harmful impacts on liver functions, resulting in elevated activities of serum ALT and AST enzymes (P < 0.05). Also, lipid stores (cholesterol, LDL, HDL, and triglyceride) were depleted due to chronic high density stress. Overall, high density (50 kg/m3) negatively influenced fish performance, but moderate culture densities (20, 30, and 40 kg/m3) do not influence growth and stress, innate immune, and metabolic responses of fish. Thus, triploid rainbow trout juveniles can act well and be recommended to the aquaculture industry at a culture density of 40 kg/m3. |
| format | Article |
| id | doaj-art-b41b0f493a7b40c692146b0c080af2ae |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-b41b0f493a7b40c692146b0c080af2ae2025-02-06T05:12:04ZengElsevierAquaculture Reports2352-51342025-03-0140102540Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss)Roghayeh Mahmoudi0Mahdi Naderi1Hakimeh Dopeikar2Seyed Ali Khorasaninasab3Seyed Hadi Seyedalhosseini4Shahid Motahary Cold-Water Fishes Genetic and Breeding Research Center, Iranian Fisheries Sciences Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Yasouj, IranDepartment of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan, Iran; Correspondence to: Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan 6361993716, Iran.Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, IranDepartment of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan, IranDepartment of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khuzestan, IranThis experiment aimed to evaluate the effects of triploidization and culture densities on the performance of rainbow trout. Juveniles (30.70 ± 1.04 g) were randomly stocked into 24 fiberglass tanks with a flow-through system. The experiment was designed with two ploidy conditions (diploid and triploid) each with 20 (40 fish/tank), 30 (59 fish/tank), 40 (79 fish/tank), and 50 (99 fish/tank) kg/m3 culture densities, a 2 × 4 factorial design. After 9 weeks, final weight, weight gain, specific growth rate, and protein efficiency ratio (PER) in fish cultured at 20, 30, and 40 kg/m3 were significantly higher and feed conversion ratio (FCR) was lower compared to those cultured at 50 kg/m3 (P < 0.05). Also, at 50 kg/m3 density, FCR in triploids was significantly higher and PER was lower compared to diploids (P < 0.05). Serum cortisol levels were highest and osmolality and total antioxidant capacity values were lowest in triploid fish cultured at the highest (50 kg/m3) density, indicating that triploids were affected more than diploids by chronic high density stress. Serum lysozyme and alternative complement (ACH50) activities and globulin content were significantly lower in fish cultured at 50 kg/m3 (P < 0.05). Thus, rainbow trout chronically exposed to high culture density (50 kg/m3) showed signs of immunosuppression. Chronic high density stress exhibited harmful impacts on liver functions, resulting in elevated activities of serum ALT and AST enzymes (P < 0.05). Also, lipid stores (cholesterol, LDL, HDL, and triglyceride) were depleted due to chronic high density stress. Overall, high density (50 kg/m3) negatively influenced fish performance, but moderate culture densities (20, 30, and 40 kg/m3) do not influence growth and stress, innate immune, and metabolic responses of fish. Thus, triploid rainbow trout juveniles can act well and be recommended to the aquaculture industry at a culture density of 40 kg/m3.http://www.sciencedirect.com/science/article/pii/S2352513424006288TriploidizationStocking densityChronic stressImmunityTriploid rainbow trout |
| spellingShingle | Roghayeh Mahmoudi Mahdi Naderi Hakimeh Dopeikar Seyed Ali Khorasaninasab Seyed Hadi Seyedalhosseini Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss) Aquaculture Reports Triploidization Stocking density Chronic stress Immunity Triploid rainbow trout |
| title | Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss) |
| title_full | Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss) |
| title_fullStr | Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss) |
| title_full_unstemmed | Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss) |
| title_short | Interactive effects of triploidy induction and culture densities on growth performance and stress, immune, and metabolic responses in juvenile rainbow trout (Oncorhynchus mykiss) |
| title_sort | interactive effects of triploidy induction and culture densities on growth performance and stress immune and metabolic responses in juvenile rainbow trout oncorhynchus mykiss |
| topic | Triploidization Stocking density Chronic stress Immunity Triploid rainbow trout |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006288 |
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