Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering
Currently, there are no therapies that prevent the negative myocardial remodeling process that occurs after a heart attack. Injectable hydrogels are a treatment option because they may replace the damaged extracellular matrix and, in addition, can be administered minimally invasively. Reactive oxyge...
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MDPI AG
2025-01-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/11/1/53 |
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| author | José Luis Hidalgo-Vicelis Angélica Raquel Rivera-Contreras Beatriz Hernández-Téllez Gabriela Piñón-Zárate Katia Jarquín-Yáñez Tatiana Fiordelisio-Coll José Manuel Saniger-Blesa Gertrudis Hortensia González-Gómez María Alicia Falcón-Neri María Margarita Canales-Martínez Andrés Eliú Castell-Rodríguez |
| author_facet | José Luis Hidalgo-Vicelis Angélica Raquel Rivera-Contreras Beatriz Hernández-Téllez Gabriela Piñón-Zárate Katia Jarquín-Yáñez Tatiana Fiordelisio-Coll José Manuel Saniger-Blesa Gertrudis Hortensia González-Gómez María Alicia Falcón-Neri María Margarita Canales-Martínez Andrés Eliú Castell-Rodríguez |
| author_sort | José Luis Hidalgo-Vicelis |
| collection | DOAJ |
| description | Currently, there are no therapies that prevent the negative myocardial remodeling process that occurs after a heart attack. Injectable hydrogels are a treatment option because they may replace the damaged extracellular matrix and, in addition, can be administered minimally invasively. Reactive oxygen species generated by ischemia-reperfusion damage can limit the therapeutic efficacy of injectable hydrogels. In order to overcome this limitation, grape seed proanthocyanidins were incorporated as antioxidant compounds into a thermosensitive myocardial extracellular matrix hydrogel in this study. For the fabrication of the hydrogel, the extracellular matrix obtained by decellularization of porcine myocardium was solubilized through enzymatic digestion, and the proanthocyanidins were incorporated. After exposing this extracellular matrix solution to 37 °C, it self-assembled into a hydrogel with a porous structure. According to the physicochemical and biological evaluation, the coupling of proanthocyanidins in the hydrogel has a positive effect on the antioxidant capacity, gelation kinetics, in vitro degradation, and cardiomyocyte viability, indicating that the hydrogel coupled with this type of antioxidants represents a promising alternative for potential application in post-infarction myocardial regeneration. Furthermore, this study proposes the best concentrations of proanthocyanidins that resulted in the hydrogels for future studies in cardiac tissue engineering. |
| format | Article |
| id | doaj-art-b3ff4c8e19a340e8a2f41fdb0f821562 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-b3ff4c8e19a340e8a2f41fdb0f8215622025-01-24T13:33:55ZengMDPI AGGels2310-28612025-01-011115310.3390/gels11010053Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue EngineeringJosé Luis Hidalgo-Vicelis0Angélica Raquel Rivera-Contreras1Beatriz Hernández-Téllez2Gabriela Piñón-Zárate3Katia Jarquín-Yáñez4Tatiana Fiordelisio-Coll5José Manuel Saniger-Blesa6Gertrudis Hortensia González-Gómez7María Alicia Falcón-Neri8María Margarita Canales-Martínez9Andrés Eliú Castell-Rodríguez10Laboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Comparative Neuroendocrinology, Department of Biology, Faculty of Sciences, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoGroup of Nanostructured Supports, Department of Micro and Nanotechnologies, Institute of Applied Sciences and Technology, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Functional Biophysics, Department of Physics, Faculty of Sciences, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Functional Biophysics, Department of Physics, Faculty of Sciences, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoLaboratory of Pharmacognosy, Unit of Biotechnology and Prototypes, Faculty of Higher Studies Iztacala, National Autonomous University of Mexico, Avenida de los Barrios 1, Los Reyes Iztacala, Tlalnepantla de Baz 54090, Estado de México, MexicoLaboratory of Immunotherapy and Tissue Engineering, Department of Cellular and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Av. Universidad 3000, Copilco Universidad, Coyoacán, Ciudad de México 04510, MexicoCurrently, there are no therapies that prevent the negative myocardial remodeling process that occurs after a heart attack. Injectable hydrogels are a treatment option because they may replace the damaged extracellular matrix and, in addition, can be administered minimally invasively. Reactive oxygen species generated by ischemia-reperfusion damage can limit the therapeutic efficacy of injectable hydrogels. In order to overcome this limitation, grape seed proanthocyanidins were incorporated as antioxidant compounds into a thermosensitive myocardial extracellular matrix hydrogel in this study. For the fabrication of the hydrogel, the extracellular matrix obtained by decellularization of porcine myocardium was solubilized through enzymatic digestion, and the proanthocyanidins were incorporated. After exposing this extracellular matrix solution to 37 °C, it self-assembled into a hydrogel with a porous structure. According to the physicochemical and biological evaluation, the coupling of proanthocyanidins in the hydrogel has a positive effect on the antioxidant capacity, gelation kinetics, in vitro degradation, and cardiomyocyte viability, indicating that the hydrogel coupled with this type of antioxidants represents a promising alternative for potential application in post-infarction myocardial regeneration. Furthermore, this study proposes the best concentrations of proanthocyanidins that resulted in the hydrogels for future studies in cardiac tissue engineering.https://www.mdpi.com/2310-2861/11/1/53hydrogelextracellular matrixgrape seed proanthocyanidinscardiac tissue engineering |
| spellingShingle | José Luis Hidalgo-Vicelis Angélica Raquel Rivera-Contreras Beatriz Hernández-Téllez Gabriela Piñón-Zárate Katia Jarquín-Yáñez Tatiana Fiordelisio-Coll José Manuel Saniger-Blesa Gertrudis Hortensia González-Gómez María Alicia Falcón-Neri María Margarita Canales-Martínez Andrés Eliú Castell-Rodríguez Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering Gels hydrogel extracellular matrix grape seed proanthocyanidins cardiac tissue engineering |
| title | Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering |
| title_full | Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering |
| title_fullStr | Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering |
| title_full_unstemmed | Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering |
| title_short | Thermosensitive Porcine Myocardial Extracellular Matrix Hydrogel Coupled with Proanthocyanidins for Cardiac Tissue Engineering |
| title_sort | thermosensitive porcine myocardial extracellular matrix hydrogel coupled with proanthocyanidins for cardiac tissue engineering |
| topic | hydrogel extracellular matrix grape seed proanthocyanidins cardiac tissue engineering |
| url | https://www.mdpi.com/2310-2861/11/1/53 |
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