The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro
The effect of lyso-PAF on ciliated cells was investigated in vitro. Normal mucosa was surgically obtained from human paranasal sinuses and incubated in the form of tissue culture. Ciliated epithelium was magnified under an inverted microscope, and ciliary movement was photo-electrically measured. Ci...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000074 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The effect of lyso-PAF on ciliated cells was investigated in vitro.
Normal mucosa was surgically obtained from human paranasal sinuses
and incubated in the form of tissue culture. Ciliated epithelium was
magnified under an inverted microscope, and ciliary movement was
photo-electrically measured. Ciliary activity was significantly
inhibited by 10−8 M lyso-PAF and could be restored. The effect of
lyso-PAF was completely blocked by CV-6209, a specific PAF
antagonist. The PAF concentration in the incubation medium of
lyso-PAF was determined by radioimmunoassay, because PAF is a well
known inhibitor of ciliary activity. PAF gradually increased and
after 20 min reached its maximal level. These findings indicated the
existence of an enzyme in the paranasal sinus mucosa, by which
lyso-PAF is converted to PAF, and that lyso-PAF can inhibit ciliary
activity by means of PAF. |
|---|---|
| ISSN: | 0962-9351 1466-1861 |