Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity
We encountered five cases that exhibited false-high Hemoglobin A1c (HbA1c) levels when samples were examined using the enzyme-based NORUDIA N HbA1c kit. HbA1c levels were higher than those obtained using other methods, such as HPLC, immune-based methods, and other enzyme-based kits. This kit produce...
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The Japan Endocrine Society
2024-01-01
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| Series: | Endocrine Journal |
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| Online Access: | https://www.jstage.jst.go.jp/article/endocrj/71/1/71_EJ23-0212/_html/-char/en |
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| author | Kenji Hara Atsushi Ujiie Shiori Suzuki Takenori Okumura Mio Kubo Hiroyuki Shinozaki Mototaka Yamauchi Takafumi Tsuchiya Kohzo Takebayashi Nobuyuki Shimozawa Masafumi Koga Koshi Hashimoto |
| author_facet | Kenji Hara Atsushi Ujiie Shiori Suzuki Takenori Okumura Mio Kubo Hiroyuki Shinozaki Mototaka Yamauchi Takafumi Tsuchiya Kohzo Takebayashi Nobuyuki Shimozawa Masafumi Koga Koshi Hashimoto |
| author_sort | Kenji Hara |
| collection | DOAJ |
| description | We encountered five cases that exhibited false-high Hemoglobin A1c (HbA1c) levels when samples were examined using the enzyme-based NORUDIA N HbA1c kit. HbA1c levels were higher than those obtained using other methods, such as HPLC, immune-based methods, and other enzyme-based kits. This kit produced inaccurate results for HbA1c when residual peroxides were present in samples. The addition of peroxidase solution restored false-high HbA1c levels in the five cases, indicating that reduced catalase activity was responsible for these values because catalase eliminates peroxide. Catalase activity and gene mutations were examined in the five cases and an immunohistological analysis was performed to assess the expression of catalase. Cases #1 and 2 were diagnosed as acatalasemia and cases #3, 4, and 5 as hypocatalasemia based on compound heterozygous SNP and heterozygous splicing mutations in the catalase gene. Therefore, impaired catalase activity was responsible for false-high HbA1c levels measured by the NORUDIA N HbA1c kit. |
| format | Article |
| id | doaj-art-b3b4a1d0be744d8d9cfaea192bfa0853 |
| institution | Kabale University |
| issn | 1348-4540 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | The Japan Endocrine Society |
| record_format | Article |
| series | Endocrine Journal |
| spelling | doaj-art-b3b4a1d0be744d8d9cfaea192bfa08532025-01-22T06:31:09ZengThe Japan Endocrine SocietyEndocrine Journal1348-45402024-01-01711394410.1507/endocrj.EJ23-0212endocrjAnalysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activityKenji Hara0Atsushi Ujiie1Shiori Suzuki2Takenori Okumura3Mio Kubo4Hiroyuki Shinozaki5Mototaka Yamauchi6Takafumi Tsuchiya7Kohzo Takebayashi8Nobuyuki Shimozawa9Masafumi Koga10Koshi Hashimoto11Department of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanDivision of Genomics Research, Life Science Research Center, Gifu University, Gifu 501-1193, JapanDepartment of Internal Medicine, Hakuhokai Central Hospital, Hyogo 661-0953, JapanDepartment of Diabetes, Endocrinology and Hematology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, JapanWe encountered five cases that exhibited false-high Hemoglobin A1c (HbA1c) levels when samples were examined using the enzyme-based NORUDIA N HbA1c kit. HbA1c levels were higher than those obtained using other methods, such as HPLC, immune-based methods, and other enzyme-based kits. This kit produced inaccurate results for HbA1c when residual peroxides were present in samples. The addition of peroxidase solution restored false-high HbA1c levels in the five cases, indicating that reduced catalase activity was responsible for these values because catalase eliminates peroxide. Catalase activity and gene mutations were examined in the five cases and an immunohistological analysis was performed to assess the expression of catalase. Cases #1 and 2 were diagnosed as acatalasemia and cases #3, 4, and 5 as hypocatalasemia based on compound heterozygous SNP and heterozygous splicing mutations in the catalase gene. Therefore, impaired catalase activity was responsible for false-high HbA1c levels measured by the NORUDIA N HbA1c kit.https://www.jstage.jst.go.jp/article/endocrj/71/1/71_EJ23-0212/_html/-char/enhemoglobin a1c (hba1c)false-highcatalaseacatalasemiahypocatalasemia |
| spellingShingle | Kenji Hara Atsushi Ujiie Shiori Suzuki Takenori Okumura Mio Kubo Hiroyuki Shinozaki Mototaka Yamauchi Takafumi Tsuchiya Kohzo Takebayashi Nobuyuki Shimozawa Masafumi Koga Koshi Hashimoto Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity Endocrine Journal hemoglobin a1c (hba1c) false-high catalase acatalasemia hypocatalasemia |
| title | Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity |
| title_full | Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity |
| title_fullStr | Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity |
| title_full_unstemmed | Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity |
| title_short | Analysis of five cases showing false-high Hemoglobin A1c due to reduced catalase activity |
| title_sort | analysis of five cases showing false high hemoglobin a1c due to reduced catalase activity |
| topic | hemoglobin a1c (hba1c) false-high catalase acatalasemia hypocatalasemia |
| url | https://www.jstage.jst.go.jp/article/endocrj/71/1/71_EJ23-0212/_html/-char/en |
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