Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus
Introduction. Systemic lupus erythematosus (SLE) is one of the systemic autoimmune diseases characterized by the polyclonal autoantibody production. The human homologue of the mouse double minute 2 (MDM2) is well known as the negative regulator of p53. MDM2 has been reported to be overexpressed in S...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/963568 |
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| author | Yuan Liu Liping Dai Weihong Liu Guixiu Shi Jianying Zhang |
| author_facet | Yuan Liu Liping Dai Weihong Liu Guixiu Shi Jianying Zhang |
| author_sort | Yuan Liu |
| collection | DOAJ |
| description | Introduction. Systemic lupus erythematosus (SLE) is one of the systemic autoimmune diseases characterized by the polyclonal autoantibody production. The human homologue of the mouse double minute 2 (MDM2) is well known as the negative regulator of p53. MDM2 has been reported to be overexpressed in SLE animal model and to promote SLE. Since abnormally expressed proteins can induce autoimmune response, anti-MDM2 autoantibody was examined in SLE patients. Methods. Anti-MDM2 antibody in sera from 43 SLE patients and 69 healthy persons was investigated by ELISA. Positive samples were further confirmed by western blotting. The immunological feathers of anti-MDM2 positive sera were analyzed by indirect immunofluorescence assay. Anti-p53 was also investigated in SLE patients by ELISA, and the correlation of anti-MDM2 and anti-p53 was analyzed. Results. The presence of anti-MDM2 in SLE patients was 23.30%, much higher than normal healthy persons (4.30%). These anti-MDM2 positive sera present a nuclear staining pattern. The presence of anti-p53 in SLE patients was 39.50%, and the titer of anti-MDM2 was positively correlated with anti-p53 in SLE patients. Conclusions. Anti-MDM2 autoantibody was detected at high prevalence in SLE patients. The detection of anti-MDM2 in SLE patients should be clinically useful. |
| format | Article |
| id | doaj-art-b3638879d3514951bb2e9c64ad1b6c75 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-b3638879d3514951bb2e9c64ad1b6c752025-02-03T01:28:04ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/963568963568Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus ErythematosusYuan Liu0Liping Dai1Weihong Liu2Guixiu Shi3Jianying Zhang4Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital, Xiamen University, No. 55, Zhenhai Road, Xiamen 361003, ChinaDepartment of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USADepartment of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USADepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital, Xiamen University, No. 55, Zhenhai Road, Xiamen 361003, ChinaDepartment of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USAIntroduction. Systemic lupus erythematosus (SLE) is one of the systemic autoimmune diseases characterized by the polyclonal autoantibody production. The human homologue of the mouse double minute 2 (MDM2) is well known as the negative regulator of p53. MDM2 has been reported to be overexpressed in SLE animal model and to promote SLE. Since abnormally expressed proteins can induce autoimmune response, anti-MDM2 autoantibody was examined in SLE patients. Methods. Anti-MDM2 antibody in sera from 43 SLE patients and 69 healthy persons was investigated by ELISA. Positive samples were further confirmed by western blotting. The immunological feathers of anti-MDM2 positive sera were analyzed by indirect immunofluorescence assay. Anti-p53 was also investigated in SLE patients by ELISA, and the correlation of anti-MDM2 and anti-p53 was analyzed. Results. The presence of anti-MDM2 in SLE patients was 23.30%, much higher than normal healthy persons (4.30%). These anti-MDM2 positive sera present a nuclear staining pattern. The presence of anti-p53 in SLE patients was 39.50%, and the titer of anti-MDM2 was positively correlated with anti-p53 in SLE patients. Conclusions. Anti-MDM2 autoantibody was detected at high prevalence in SLE patients. The detection of anti-MDM2 in SLE patients should be clinically useful.http://dx.doi.org/10.1155/2015/963568 |
| spellingShingle | Yuan Liu Liping Dai Weihong Liu Guixiu Shi Jianying Zhang Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus Journal of Immunology Research |
| title | Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus |
| title_full | Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus |
| title_fullStr | Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus |
| title_full_unstemmed | Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus |
| title_short | Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus |
| title_sort | autoantibody to mdm2 a potential serological marker of systemic lupus erythematosus |
| url | http://dx.doi.org/10.1155/2015/963568 |
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