Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture
Examination of the virus-cell interactions is of both scientific and practical importance. Our study was aimed at comparative characterization of rabbit myxoma virus and Shope fibroma virus biological properties that manifested during the virus reproduction in RK-13/2-03 clonal continuous rabbit kid...
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Da Vinci Media
2021-11-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/594 |
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| author | O. G. Lapteva S. G. Yurkov I. P. Sindryakova A. V. Lunitsin |
| author_facet | O. G. Lapteva S. G. Yurkov I. P. Sindryakova A. V. Lunitsin |
| author_sort | O. G. Lapteva |
| collection | DOAJ |
| description | Examination of the virus-cell interactions is of both scientific and practical importance. Our study was aimed at comparative characterization of rabbit myxoma virus and Shope fibroma virus biological properties that manifested during the virus reproduction in RK-13/2-03 clonal continuous rabbit kidney cell culture. It was demonstrated that the viruses varied in infection development and cytopathic effect duration in RK-13/2-03 cell culture. Apparent lesions in cell monolayers infected by myxoma virus and fibroma virus at similar multiplicity of infection and cultivation temperature were observed on day 2 and day 3 of cultivation, respec- tively, as well as maximum cell lesions with evident degeneration were observed on day 3 and day 6 of cultivation, respectively. Myxoma virus was accumulated at titre of 6.25–6.50 lg TCID50/0,2 cm3, and Shope fibroma virusа was accumulated at titre of 5.50−5.75 lg TCID50/0.2 cm3. Shope fibroma virus demonstrated such infectivity during three passages and myxoma virus demonstrated such infectivity during twenty passages. Prepared cultures were identified as myxoma virus and Shope fibroma virus with molecular genetic analysis. Tests of the viruses for their antigenic relatedness showed that antibodies against myxoma virus were able to neutralize Shope fibroma virus also. NT titres of antibodies against both viruses were similar (1:8). RK-13/2-03 cell culture was found to be highly permissive to Shope fibroma virus that had been isolated from the diseased rabbit and not been an attenuated variant. |
| format | Article |
| id | doaj-art-b32c7b9a3a57451b8290c669fc9c3f32 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2021-11-01 |
| publisher | Da Vinci Media |
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| series | Ветеринария сегодня |
| spelling | doaj-art-b32c7b9a3a57451b8290c669fc9c3f322025-02-06T09:52:09ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592021-11-0110432332810.29326/2304-196X-2021-10-4-323-328537Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell cultureO. G. Lapteva0S. G. Yurkov1I. P. Sindryakova2A. V. Lunitsin3Federal Research Center for Virology and MicrobiologyFederal Research Center for Virology and MicrobiologyFederal Research Center for Virology and MicrobiologyFederal Research Center for Virology and MicrobiologyExamination of the virus-cell interactions is of both scientific and practical importance. Our study was aimed at comparative characterization of rabbit myxoma virus and Shope fibroma virus biological properties that manifested during the virus reproduction in RK-13/2-03 clonal continuous rabbit kidney cell culture. It was demonstrated that the viruses varied in infection development and cytopathic effect duration in RK-13/2-03 cell culture. Apparent lesions in cell monolayers infected by myxoma virus and fibroma virus at similar multiplicity of infection and cultivation temperature were observed on day 2 and day 3 of cultivation, respec- tively, as well as maximum cell lesions with evident degeneration were observed on day 3 and day 6 of cultivation, respectively. Myxoma virus was accumulated at titre of 6.25–6.50 lg TCID50/0,2 cm3, and Shope fibroma virusа was accumulated at titre of 5.50−5.75 lg TCID50/0.2 cm3. Shope fibroma virus demonstrated such infectivity during three passages and myxoma virus demonstrated such infectivity during twenty passages. Prepared cultures were identified as myxoma virus and Shope fibroma virus with molecular genetic analysis. Tests of the viruses for their antigenic relatedness showed that antibodies against myxoma virus were able to neutralize Shope fibroma virus also. NT titres of antibodies against both viruses were similar (1:8). RK-13/2-03 cell culture was found to be highly permissive to Shope fibroma virus that had been isolated from the diseased rabbit and not been an attenuated variant.https://veterinary.arriah.ru/jour/article/view/594continuous cell culturemyxoma virusshope fibroma virusvirus-specific cytopathic effect |
| spellingShingle | O. G. Lapteva S. G. Yurkov I. P. Sindryakova A. V. Lunitsin Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture Ветеринария сегодня continuous cell culture myxoma virus shope fibroma virus virus-specific cytopathic effect |
| title | Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture |
| title_full | Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture |
| title_fullStr | Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture |
| title_full_unstemmed | Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture |
| title_short | Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture |
| title_sort | comparative characterization of leporipoxvirus members reproduction in continuous cell culture |
| topic | continuous cell culture myxoma virus shope fibroma virus virus-specific cytopathic effect |
| url | https://veterinary.arriah.ru/jour/article/view/594 |
| work_keys_str_mv | AT oglapteva comparativecharacterizationofleporipoxvirusmembersreproductionincontinuouscellculture AT sgyurkov comparativecharacterizationofleporipoxvirusmembersreproductionincontinuouscellculture AT ipsindryakova comparativecharacterizationofleporipoxvirusmembersreproductionincontinuouscellculture AT avlunitsin comparativecharacterizationofleporipoxvirusmembersreproductionincontinuouscellculture |