Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral Hemorrhage
High-mobility group protein box-1 (HMGB1) is a proinflammatory involved in many inflammatory diseases. However, its roles in intracerebral hemorrhage (ICH) remain unknown. The purpose of this study was to examine the correlation between changes in serum levels of HMGB1 following acute ICH and the se...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2010/142458 |
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| author | Yu Zhou Kun-Lin Xiong Sen Lin Qi Zhong Feng-Lin Lu Hong Liang Jing-Cheng Li Jing-Zhou Wang Qing-Wu Yang |
| author_facet | Yu Zhou Kun-Lin Xiong Sen Lin Qi Zhong Feng-Lin Lu Hong Liang Jing-Cheng Li Jing-Zhou Wang Qing-Wu Yang |
| author_sort | Yu Zhou |
| collection | DOAJ |
| description | High-mobility group protein box-1 (HMGB1) is a proinflammatory involved in many inflammatory diseases. However, its roles in intracerebral hemorrhage (ICH) remain unknown. The purpose of this study was to examine the correlation between changes in serum levels of HMGB1 following acute ICH and the severity of stroke as well as the underlying mechanism. Changes in serum levels of HMGB1 in 60 consecutive patients with primary hemispheric ICH within 12 hours of onset of symptoms were determined. The correlation of HMGB1 with disease severity, IL-6, and TNF-α was analyzed. Changes in HMGB1 levels were detected with ELISA and Western blot. Compared with normal controls, patients with ICH had markedly elevated levels of HMGB1, which was significantly correlated with the levels of IL-6 and TNF-α, NIHSS score at the 10th day, and mRS score at 3 months. In comparison with the control group, the levels of HMGB1 in the perihematomal tissue in mice with ICH increased dramatically, peaked at 72 hours, and decreased at 5 days. Meanwhile, heme could stimulate cultured microglia to release large amounts of HMGB1 whereas Fe2+/3+ ions failed to stimulate HMGB1 production from microglia. Our findings suggest that HMGB1 may play an essential role in the ICH-caused inflammatory injury. |
| format | Article |
| id | doaj-art-b131a85c2c2445ee8b6cd7254e97a802 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b131a85c2c2445ee8b6cd7254e97a8022025-02-03T01:01:56ZengWileyMediators of Inflammation0962-93511466-18612010-01-01201010.1155/2010/142458142458Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral HemorrhageYu Zhou0Kun-Lin Xiong1Sen Lin2Qi Zhong3Feng-Lin Lu4Hong Liang5Jing-Cheng Li6Jing-Zhou Wang7Qing-Wu Yang8Department of Neurology, Daping Hospital, The Third Military Medical University, Changjiang Branch Road no. 10, Yuzhong District, Chongqing 400042, ChinaDepartment of Radiology, Daping Hospital, The Third Military Medical University, Yuzhong District, Chongqing 400042, ChinaDepartment of Neurology, Daping Hospital, The Third Military Medical University, Changjiang Branch Road no. 10, Yuzhong District, Chongqing 400042, ChinaDepartment of Neurology, Daping Hospital, The Third Military Medical University, Changjiang Branch Road no. 10, Yuzhong District, Chongqing 400042, ChinaCollege of Biomedical Engineering, Chongqing University, Chongqing 400044, ChinaDepartment of Neurosurgery, Daping Hospital, The Third Military Medical University, Yuzhong District, Chongqing 400042, ChinaDepartment of Neurology, Daping Hospital, The Third Military Medical University, Changjiang Branch Road no. 10, Yuzhong District, Chongqing 400042, ChinaDepartment of Neurology, Daping Hospital, The Third Military Medical University, Changjiang Branch Road no. 10, Yuzhong District, Chongqing 400042, ChinaDepartment of Neurology, Daping Hospital, The Third Military Medical University, Changjiang Branch Road no. 10, Yuzhong District, Chongqing 400042, ChinaHigh-mobility group protein box-1 (HMGB1) is a proinflammatory involved in many inflammatory diseases. However, its roles in intracerebral hemorrhage (ICH) remain unknown. The purpose of this study was to examine the correlation between changes in serum levels of HMGB1 following acute ICH and the severity of stroke as well as the underlying mechanism. Changes in serum levels of HMGB1 in 60 consecutive patients with primary hemispheric ICH within 12 hours of onset of symptoms were determined. The correlation of HMGB1 with disease severity, IL-6, and TNF-α was analyzed. Changes in HMGB1 levels were detected with ELISA and Western blot. Compared with normal controls, patients with ICH had markedly elevated levels of HMGB1, which was significantly correlated with the levels of IL-6 and TNF-α, NIHSS score at the 10th day, and mRS score at 3 months. In comparison with the control group, the levels of HMGB1 in the perihematomal tissue in mice with ICH increased dramatically, peaked at 72 hours, and decreased at 5 days. Meanwhile, heme could stimulate cultured microglia to release large amounts of HMGB1 whereas Fe2+/3+ ions failed to stimulate HMGB1 production from microglia. Our findings suggest that HMGB1 may play an essential role in the ICH-caused inflammatory injury.http://dx.doi.org/10.1155/2010/142458 |
| spellingShingle | Yu Zhou Kun-Lin Xiong Sen Lin Qi Zhong Feng-Lin Lu Hong Liang Jing-Cheng Li Jing-Zhou Wang Qing-Wu Yang Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral Hemorrhage Mediators of Inflammation |
| title | Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral Hemorrhage |
| title_full | Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral Hemorrhage |
| title_fullStr | Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral Hemorrhage |
| title_full_unstemmed | Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral Hemorrhage |
| title_short | Elevation of High-Mobility Group Protein Box-1 in Serum Correlates with Severity of Acute Intracerebral Hemorrhage |
| title_sort | elevation of high mobility group protein box 1 in serum correlates with severity of acute intracerebral hemorrhage |
| url | http://dx.doi.org/10.1155/2010/142458 |
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