Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines
This study was aimed to collect Pleurotus eryngii (DC.) Quél. edible/medicinal mushroom from Çanakkale/Kumkale and to evaluate the antiproliferative effects of alcoholic extracts on breast and colon cancer cell lines. For this purpose, the Soxhlet method prepared methanol and ethanol:H2O (70:30) ext...
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Çanakkale Onsekiz Mart University
2024-09-01
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| Series: | Journal of Advanced Research in Natural and Applied Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3999258 |
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| author | Mehmet Ay Hakan Allı Alper Önder Gülce Davutlar Ferah Cömert Önder |
| author_facet | Mehmet Ay Hakan Allı Alper Önder Gülce Davutlar Ferah Cömert Önder |
| author_sort | Mehmet Ay |
| collection | DOAJ |
| description | This study was aimed to collect Pleurotus eryngii (DC.) Quél. edible/medicinal mushroom from Çanakkale/Kumkale and to evaluate the antiproliferative effects of alcoholic extracts on breast and colon cancer cell lines. For this purpose, the Soxhlet method prepared methanol and ethanol:H2O (70:30) extracts by gradually increasing solvent polarities. Then, breast (MDA-MB-231, BT-549, BT-20, MCF-7) and colon (HT-29) cancer cell lines were treated with the extracts in increasing concentrations (0.05-0.5 mg/mL) for 48 h. In conclusion, methanol and aqueous ethanol extracts exhibited significant antiproliferative effects in cancer cell lines, according to the MTT assay. The cell viability in the triple-negative breast cancer (TNBC) MDA-MB-231 cell line was highly decreased by methanol extract at a very low concentration (0.1 mg/mL). Furthermore, methanol extract at 0.3 mg/mL reduced the percentage of cell viability in the HT-29 cell line. Aqueous ethanol extract showed antiproliferative activity in breast and colon cancer cell lines at 0.25 mg/mL concentration for 48 h applications. In addition, some bioactive components such as 4-hydroxy flavone, chrysin, and tannic acid of aqueous ethanol extract have been determined by High-Performance Liquid Chromatography (HPLC) analysis. As a result, this study may increase interest in the studies on the anti-cancer activities and the mechanisms of P. eryngii mushroom. |
| format | Article |
| id | doaj-art-b1089738b33441359aca774f7bfdb702 |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-b1089738b33441359aca774f7bfdb7022025-02-05T18:13:03ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952024-09-0110365966810.28979/jarnas.1499726453Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell LinesMehmet Ay0https://orcid.org/0000-0002-1095-1614Hakan Allı1https://orcid.org/0000-0001-8781-7029Alper Önder2https://orcid.org/0000-0002-0775-0053Gülce Davutlar3https://orcid.org/0009-0004-3970-0546Ferah Cömert Önder4https://orcid.org/0000-0002-4037-1979ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİMUĞLA SITKI KOÇMAN ÜNİVERSİTESİÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, TIP FAKÜLTESİThis study was aimed to collect Pleurotus eryngii (DC.) Quél. edible/medicinal mushroom from Çanakkale/Kumkale and to evaluate the antiproliferative effects of alcoholic extracts on breast and colon cancer cell lines. For this purpose, the Soxhlet method prepared methanol and ethanol:H2O (70:30) extracts by gradually increasing solvent polarities. Then, breast (MDA-MB-231, BT-549, BT-20, MCF-7) and colon (HT-29) cancer cell lines were treated with the extracts in increasing concentrations (0.05-0.5 mg/mL) for 48 h. In conclusion, methanol and aqueous ethanol extracts exhibited significant antiproliferative effects in cancer cell lines, according to the MTT assay. The cell viability in the triple-negative breast cancer (TNBC) MDA-MB-231 cell line was highly decreased by methanol extract at a very low concentration (0.1 mg/mL). Furthermore, methanol extract at 0.3 mg/mL reduced the percentage of cell viability in the HT-29 cell line. Aqueous ethanol extract showed antiproliferative activity in breast and colon cancer cell lines at 0.25 mg/mL concentration for 48 h applications. In addition, some bioactive components such as 4-hydroxy flavone, chrysin, and tannic acid of aqueous ethanol extract have been determined by High-Performance Liquid Chromatography (HPLC) analysis. As a result, this study may increase interest in the studies on the anti-cancer activities and the mechanisms of P. eryngii mushroom.https://dergipark.org.tr/en/download/article-file/3999258pleurotus eryngii (dc.) quél.mushroomantiproliferationnatural productscancer |
| spellingShingle | Mehmet Ay Hakan Allı Alper Önder Gülce Davutlar Ferah Cömert Önder Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines Journal of Advanced Research in Natural and Applied Sciences pleurotus eryngii (dc.) quél. mushroom antiproliferation natural products cancer |
| title | Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines |
| title_full | Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines |
| title_fullStr | Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines |
| title_full_unstemmed | Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines |
| title_short | Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines |
| title_sort | effects of pleurotus eryngii dc quel mushroom extracts on cell proliferation in breast and colon cancer cell lines |
| topic | pleurotus eryngii (dc.) quél. mushroom antiproliferation natural products cancer |
| url | https://dergipark.org.tr/en/download/article-file/3999258 |
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