Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis

This work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-...

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Main Authors: Kabkaew L. Sukontason, Tarinee Chaiwong, Urai Chaisri, Hiromu Kurahashi, Michelle Sanford, Kom Sukontason
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Journal of Parasitology Research
Online Access:http://dx.doi.org/10.1155/2011/690863
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author Kabkaew L. Sukontason
Tarinee Chaiwong
Urai Chaisri
Hiromu Kurahashi
Michelle Sanford
Kom Sukontason
author_facet Kabkaew L. Sukontason
Tarinee Chaiwong
Urai Chaisri
Hiromu Kurahashi
Michelle Sanford
Kom Sukontason
author_sort Kabkaew L. Sukontason
collection DOAJ
description This work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-day-old flies and observed under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM displayed a smooth surface which is occasionally penetrated by tracheoles. TEM of the testis in the pupal stage presents the thick testis wall covering underdeveloped cells containing a variable size of an electron-dense globule. For the 3-day-old males, the testicular wall is formed by an external layer, a peritoneal sheath, a muscular layer, a basement membrane, and a follicular epithelium. Follicular epithelium presented developing spermatozoa. Regarding the 7-day-old males, development of spermatozoa is apparent, displaying nucleus, centriolar adjunct, axoneme, and mitochondrial derivatives, with the 9 + 9 + 2 microtubule pattern of axoneme.
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id doaj-art-b0d5ea9529c34fd4b9e776b99e178b36
institution Kabale University
issn 2090-0023
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language English
publishDate 2011-01-01
publisher Wiley
record_format Article
series Journal of Parasitology Research
spelling doaj-art-b0d5ea9529c34fd4b9e776b99e178b362025-02-03T01:26:03ZengWileyJournal of Parasitology Research2090-00232090-00312011-01-01201110.1155/2011/690863690863Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of TestisKabkaew L. Sukontason0Tarinee Chaiwong1Urai Chaisri2Hiromu Kurahashi3Michelle Sanford4Kom Sukontason5Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, ThailandCollege of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162 8640, JapanDepartment of Entomology, Texas A&M University, TX 77845-2543, USADepartment of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, ThailandThis work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-day-old flies and observed under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM displayed a smooth surface which is occasionally penetrated by tracheoles. TEM of the testis in the pupal stage presents the thick testis wall covering underdeveloped cells containing a variable size of an electron-dense globule. For the 3-day-old males, the testicular wall is formed by an external layer, a peritoneal sheath, a muscular layer, a basement membrane, and a follicular epithelium. Follicular epithelium presented developing spermatozoa. Regarding the 7-day-old males, development of spermatozoa is apparent, displaying nucleus, centriolar adjunct, axoneme, and mitochondrial derivatives, with the 9 + 9 + 2 microtubule pattern of axoneme.http://dx.doi.org/10.1155/2011/690863
spellingShingle Kabkaew L. Sukontason
Tarinee Chaiwong
Urai Chaisri
Hiromu Kurahashi
Michelle Sanford
Kom Sukontason
Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis
Journal of Parasitology Research
title Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis
title_full Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis
title_fullStr Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis
title_full_unstemmed Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis
title_short Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis
title_sort reproductive organ of blow fly chrysomya megacephala diptera calliphoridae ultrastructural of testis
url http://dx.doi.org/10.1155/2011/690863
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