Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis
This work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-...
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Language: | English |
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Wiley
2011-01-01
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Series: | Journal of Parasitology Research |
Online Access: | http://dx.doi.org/10.1155/2011/690863 |
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author | Kabkaew L. Sukontason Tarinee Chaiwong Urai Chaisri Hiromu Kurahashi Michelle Sanford Kom Sukontason |
author_facet | Kabkaew L. Sukontason Tarinee Chaiwong Urai Chaisri Hiromu Kurahashi Michelle Sanford Kom Sukontason |
author_sort | Kabkaew L. Sukontason |
collection | DOAJ |
description | This work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-day-old flies and observed under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM displayed a smooth surface which is occasionally penetrated by tracheoles. TEM of the testis in the pupal stage presents the thick testis wall covering underdeveloped cells containing a variable size of an electron-dense globule. For the 3-day-old males, the testicular wall is formed by an external layer, a peritoneal sheath, a muscular layer, a basement membrane, and a follicular epithelium. Follicular epithelium presented developing spermatozoa. Regarding the 7-day-old males, development of spermatozoa is apparent, displaying nucleus, centriolar adjunct, axoneme, and mitochondrial derivatives, with the 9 + 9 + 2 microtubule pattern of axoneme. |
format | Article |
id | doaj-art-b0d5ea9529c34fd4b9e776b99e178b36 |
institution | Kabale University |
issn | 2090-0023 2090-0031 |
language | English |
publishDate | 2011-01-01 |
publisher | Wiley |
record_format | Article |
series | Journal of Parasitology Research |
spelling | doaj-art-b0d5ea9529c34fd4b9e776b99e178b362025-02-03T01:26:03ZengWileyJournal of Parasitology Research2090-00232090-00312011-01-01201110.1155/2011/690863690863Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of TestisKabkaew L. Sukontason0Tarinee Chaiwong1Urai Chaisri2Hiromu Kurahashi3Michelle Sanford4Kom Sukontason5Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, ThailandCollege of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, ThailandDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162 8640, JapanDepartment of Entomology, Texas A&M University, TX 77845-2543, USADepartment of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, ThailandThis work presents the ultrastructure of testis of the medically important blow fly, Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) using light microscopy and electron microscopy. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3-day-old flies and 7-day-old flies and observed under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM displayed a smooth surface which is occasionally penetrated by tracheoles. TEM of the testis in the pupal stage presents the thick testis wall covering underdeveloped cells containing a variable size of an electron-dense globule. For the 3-day-old males, the testicular wall is formed by an external layer, a peritoneal sheath, a muscular layer, a basement membrane, and a follicular epithelium. Follicular epithelium presented developing spermatozoa. Regarding the 7-day-old males, development of spermatozoa is apparent, displaying nucleus, centriolar adjunct, axoneme, and mitochondrial derivatives, with the 9 + 9 + 2 microtubule pattern of axoneme.http://dx.doi.org/10.1155/2011/690863 |
spellingShingle | Kabkaew L. Sukontason Tarinee Chaiwong Urai Chaisri Hiromu Kurahashi Michelle Sanford Kom Sukontason Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis Journal of Parasitology Research |
title | Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis |
title_full | Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis |
title_fullStr | Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis |
title_full_unstemmed | Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis |
title_short | Reproductive Organ of Blow Fly, Chrysomya megacephala (Diptera: Calliphoridae): Ultrastructural of Testis |
title_sort | reproductive organ of blow fly chrysomya megacephala diptera calliphoridae ultrastructural of testis |
url | http://dx.doi.org/10.1155/2011/690863 |
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