Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker
<b>Background/Objectives</b>: Liver injury is common after abdominal trauma. However, the established biomarkers of liver injury, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), lack accuracy. This study investigates whether specific liver-related microRNAs (...
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2025-01-01
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| author | Jason-Alexander Hörauf Amit Singh Maika Voth Hamed Moheimani Cora Rebecca Schindler Borna Relja Liudmila Leppik Ingo Marzi Dirk Henrich |
| author_facet | Jason-Alexander Hörauf Amit Singh Maika Voth Hamed Moheimani Cora Rebecca Schindler Borna Relja Liudmila Leppik Ingo Marzi Dirk Henrich |
| author_sort | Jason-Alexander Hörauf |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Liver injury is common after abdominal trauma. However, the established biomarkers of liver injury, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), lack accuracy. This study investigates whether specific liver-related microRNAs (miRNAs) are released into the circulation in trauma patients with liver injury and whether they can indicate liver damage in the early phase after major trauma. <b>Methods</b>: A retrospective analysis of prospectively collected data and blood samples from 26 trauma patients was conducted. The levels of miRNA-21-5p, -122-5p, -191-5p, -192-3p, and -212-3p were measured in patients with computed tomography-confirmed liver trauma (LT group, <i>n</i> = 12) and polytrauma patients without liver trauma (PT group, <i>n</i> = 14) upon emergency room (ER) admission, and 24 and 48 h after trauma. Additionally, liver-type fatty acid binding protein (L-FABP) was measured, as it has recently been discussed in the context of abdominal trauma. <b>Results</b>: Only miRNA-122-5p showed a significant increase in the LT group compared to the PT group, but only at the 48 h time point (<i>p</i> = 0.032). Conversely, L-FABP (<i>p</i> = 0.018) and ALT (<i>p</i> = 0.05) were significantly elevated in the LT group compared to the PT group at the time of ER admission. There was a moderate correlation between miRNA-122-5p and AIS<sub>Abdomen</sub> (<i>p</i> = 0.056) and transfused red blood cell concentrates (<i>p</i> = 0.055). L-FABP correlated strongly with the ALT levels (<i>p</i> = 0.0009) and the length of stay in the ICU (<i>p</i> = 0.0086). <b>Conclusions</b>: In this study, the liver-specific miRNA-122-5p did not effectively indicate liver injury in the early acute post-traumatic phase. Future research with a large sample size should investigate whether other miRNAs can more accurately predict liver injury and the extent of hepatocellular injury, particularly in the acute post-traumatic phase. |
| format | Article |
| id | doaj-art-b0d1daab32c7432e8b2ff26d7f6baceb |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Diagnostics |
| spelling | doaj-art-b0d1daab32c7432e8b2ff26d7f6baceb2025-01-24T13:29:01ZengMDPI AGDiagnostics2075-44182025-01-0115217910.3390/diagnostics15020179Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase MarkerJason-Alexander Hörauf0Amit Singh1Maika Voth2Hamed Moheimani3Cora Rebecca Schindler4Borna Relja5Liudmila Leppik6Ingo Marzi7Dirk Henrich8Department of Trauma Surgery and Orthopedics, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, GermanyDepartment of Trauma Surgery and Orthopedics, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, GermanyDepartment of Trauma Surgery and Orthopedics, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, GermanyPittsburgh Trauma Research Center, Division of Trauma and Acute Care Surgery, Pittsburgh, PA 15213, USADepartment of Trauma Surgery and Orthopedics, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, GermanyDepartment of Trauma, Hand, Plastic and Reconstructive Surgery, Translational and Experimental Trauma Research, University Hospital Ulm, Ulm University, 89081 Ulm, GermanyDepartment of Trauma Surgery and Orthopedics, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, GermanyDepartment of Trauma Surgery and Orthopedics, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, GermanyDepartment of Trauma Surgery and Orthopedics, University Hospital Frankfurt, Goethe University Frankfurt, 60596 Frankfurt am Main, Germany<b>Background/Objectives</b>: Liver injury is common after abdominal trauma. However, the established biomarkers of liver injury, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), lack accuracy. This study investigates whether specific liver-related microRNAs (miRNAs) are released into the circulation in trauma patients with liver injury and whether they can indicate liver damage in the early phase after major trauma. <b>Methods</b>: A retrospective analysis of prospectively collected data and blood samples from 26 trauma patients was conducted. The levels of miRNA-21-5p, -122-5p, -191-5p, -192-3p, and -212-3p were measured in patients with computed tomography-confirmed liver trauma (LT group, <i>n</i> = 12) and polytrauma patients without liver trauma (PT group, <i>n</i> = 14) upon emergency room (ER) admission, and 24 and 48 h after trauma. Additionally, liver-type fatty acid binding protein (L-FABP) was measured, as it has recently been discussed in the context of abdominal trauma. <b>Results</b>: Only miRNA-122-5p showed a significant increase in the LT group compared to the PT group, but only at the 48 h time point (<i>p</i> = 0.032). Conversely, L-FABP (<i>p</i> = 0.018) and ALT (<i>p</i> = 0.05) were significantly elevated in the LT group compared to the PT group at the time of ER admission. There was a moderate correlation between miRNA-122-5p and AIS<sub>Abdomen</sub> (<i>p</i> = 0.056) and transfused red blood cell concentrates (<i>p</i> = 0.055). L-FABP correlated strongly with the ALT levels (<i>p</i> = 0.0009) and the length of stay in the ICU (<i>p</i> = 0.0086). <b>Conclusions</b>: In this study, the liver-specific miRNA-122-5p did not effectively indicate liver injury in the early acute post-traumatic phase. Future research with a large sample size should investigate whether other miRNAs can more accurately predict liver injury and the extent of hepatocellular injury, particularly in the acute post-traumatic phase.https://www.mdpi.com/2075-4418/15/2/179liver injurymiRNAtraumainflammationbiomarkerL-FABP |
| spellingShingle | Jason-Alexander Hörauf Amit Singh Maika Voth Hamed Moheimani Cora Rebecca Schindler Borna Relja Liudmila Leppik Ingo Marzi Dirk Henrich Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker Diagnostics liver injury miRNA trauma inflammation biomarker L-FABP |
| title | Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker |
| title_full | Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker |
| title_fullStr | Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker |
| title_full_unstemmed | Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker |
| title_short | Limited Diagnostic Value of miRNAs in Early Trauma-Induced Liver Injury: Only miRNA-122 Emerges as a Late-Phase Marker |
| title_sort | limited diagnostic value of mirnas in early trauma induced liver injury only mirna 122 emerges as a late phase marker |
| topic | liver injury miRNA trauma inflammation biomarker L-FABP |
| url | https://www.mdpi.com/2075-4418/15/2/179 |
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